糖尿病大鼠在不同血糖浓度下伤口愈合情况实验研究  

Experimental Study of Wound Healing Process of Diabetic Rats under Different Blood Glucose Concentration

作　　者：曾文超[1] 巩茹[1] 郭洪敏[1] 巩超[1] 王福宁[1] 赵阳 岳震[1] Zeng Wenchao;Gong Ru;Guo Hongmin(Hand and Foot Surgery Department,Jining First People's Hospital,Jining 272000,China)

机构地区：[1]山东省济宁市第一人民医院手足踝外科,山东济宁272000

出　　处：《实用骨科杂志》2019年第2期140-142,共3页Journal of Practical Orthopaedics

基　　金：山东省医药卫生科技发展计划项目(2015WS0430)

摘　　要：目的探讨不同血糖浓度对糖尿病大鼠伤口愈合的影响。方法选取普通3～4个月龄雄性大白鼠,通过链唑霉素诱导大白鼠糖尿病模型。将诱导成功的大白鼠根据胰岛素调控血糖水平分为四组,I组:糖尿病诱导后给予胰岛素控制血糖在160～190mg/dL;Ⅱ组:糖尿病诱导后给予胰岛素控制血糖在190～240mg/dL;Ⅲ组:糖尿病诱导后给予胰岛素控制血糖在240mg/dL以上;Ⅳ组:糖尿病诱导后给予胰岛素控制血糖在160mg/dL以下。手术形成创面模型。分别于伤后0(伤后当天)、3、7、14d处死大鼠。测量伤口愈合面积,观察创面组织病理学改变。结果血糖浓度控制在160～190mg/dL时,伤口愈合速度最快,其次为血糖低于160mg/dL时,巨噬细胞无明显差异。血糖在更高水平时,伤口愈合速度明显减慢,伤口周围组织的巨噬细胞明显减少。结论糖尿病大鼠在低水平高血糖浓度下有利于伤口愈合。Objective To study the effect of blood glucose level on the wound healing of diabetic rats. Methods Diabetes mellitus model was built by streptozotocin injection in male rats aged 3~4 months.The diabetes mellitus rats were divided into four groups according to the level of blood gulcose regulated by insulin.The wound model was formed by operation.Afterwards,rats were killed at 0 (the same day of injury),3,7 and 14 days after injury.The wound healing area was measured,in combination with the observation of the pathological changes of wound tissues. Results When the blood sugar concentration was controlled at 160~190 mg/dL,the wound healing rate was the fastest,followed by at the level lower than 160 mg/dL with no significant difference in macrophages.At higher blood sugar levels,the healing rate of wounds was delayed obviously,and macrophages in surrounding tissues exhibited remarkable decrease. Conclusion It is beneficial to wound healing at low levels of high blood sugar concentration in diabetic rats.

关 键 词：糖尿病大鼠 血糖浓度 病理学改变 伤口愈合 

分 类 号：R649.9[医药卫生—外科学]