TGF-β1及NF-κB P65在妊娠期甲亢大鼠子代肾脏中的表达  

Expression of TGF-β1 and NF-κB P65 in kidney of offspring of the mice with hyperthyroidism during pregnancy

作　　者：王宏[1] 王瑞英[1] 刘志红[1] 刘静[1] 张艳荣 WANG Hong;WANG Rui-ying;LIU Zhi-hong;LIU Jing;ZHANG Yan-rong(Department of Endocrinology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Endocrinology,the Second Hospital of Shijiazhuang,Hebei Province,Shijiazhuang 050051,China)

机构地区：[1]河北医科大学第二医院内分泌科,河北石家庄050000 [2]河北省石家庄市第二医院内分泌科,河北石家庄050051

出　　处：《河北医科大学学报》2019年第3期281-285,291,共6页Journal of Hebei Medical University

基　　金：河北省科技计划项目(132777132D)

摘　　要：目的观察母鼠妊娠期合并甲状腺功能亢进症(简称甲亢)时,仔鼠肾间质中转化生长因子β1(transforming growth factorβ1,TGF-β1)及核因子κB(nuclear factor kappa B,NF-κB)P65的表达,探讨母鼠妊娠期甲亢与子鼠肾间质纤维化的关系。方法 54只60日龄的SD大鼠随机分成3组,每组18只,用不同剂量的左甲状腺素钠连续灌胃,制作甲亢模型。母鼠妊娠后继续灌胃,直至分娩。观察正常对照仔鼠组、轻度甲亢仔鼠组及重度甲亢仔鼠组肾脏病理结构和损伤情况。同时应用Western blot技术测定仔鼠肾小管间质细胞TGF-β1及胞核中NF-κB P65的表达情况。结果重度甲亢仔鼠组21d和60d血清游离三碘甲状腺原氨酸(free tri-iodothyronine,FT3)、游离甲状腺素(free thyroxine,FT4)均高于正常对照仔鼠组和轻度甲亢仔鼠组,重度甲亢仔鼠组21d和60d血清促甲状腺素(thyroid stimulating hormone,TSH)低于正常对照仔鼠组和轻度甲亢仔鼠组,各组60d血清FT3、FT4均高于21d,各组60d血清TSH均低于21d,差异有统计学意义(P<0.05);轻度甲亢仔鼠组和重度甲亢仔鼠组0d、21d和60d肾小管间质细胞中TGF-β1和NF-κB P65均高于正常对照仔鼠组,重度甲亢仔鼠组又高于轻度甲亢仔鼠组(P<0.05);3组TGF-β1和NF-κB P65于21d和60d时表达水平均高于0d,60d又高于21d,差异有统计学意义(P<0.05)。结论母鼠甲亢可引起仔鼠肾间质纤维化,且与甲亢程度有关,甲亢的早期诊断与治疗对预防子代肾间质纤维化具有重要意义。Objective To observe the expressions of transforming growth factor 1(TGF-1)and nuclear factor kappa B(NF-κB)P65 in the renal interstitium of pregnant rats with hyperthyroidism and to investigate the relationship between hyperthyroidism in pregnancy and renal interstitial fibrosis in offspring.Methods Fifty-four SD rats aged 60 days were randomly divided into 3 groups,18 rats in each group which were given continuous gavage with different doses of levothyroxine sodium to make a hyperthyroidism model.After pregnancy,the mother mice continued to be gavage until delivery.The pathological structure and damage of kidney in normal control group,mild hyperthyroidism group and severe hyperthyroidism group were observed.Western blot was also used to determine the expression of TGF-1 and NF-κB P65.Results The serum levels of free tri-iodothyronine(FT3)and free thyroxine(FT4)in the severe hyperthyroidism newborn mouse group at 21 and 60 days were higher than those in the normal control newborn mouse group and the mild hyperthyroidism newborn mouse group,and the serum levels of thyroid stimulating hormone(TSH)in the severe hyperthyroidism newborn mouse group at 21 and 60 days were lower than those in the normal control newborn mouse group and the mild hyperthyroidism newborn mouse group.FT3 and FT4 were higher on day 60 than on day 21 in each group,and TSH was lower on day 60 than on day 21 in each group,with statistically significant differences(P<0.05).TGF-1 and NF-κB P65 in renal tubular interstitial cells were higher in the mild hyperthyroidism newborn mouse group and the severe hyperthyroidism newborn mouse group than in the normal control newborn mouse group at 0d,21d and 60 d.The expression levels of TGF-1 and NF-κB P65 in the 3 groups were higher at 21d and 60d than at 0 d and higher at 60 d than at 21d,with statistically significant differences(P<0.05).Conclusion Maternal hyperthyroidism can cause renal interstitial fibrosis in offspring,which is related to the degree of hyperthyroidism.The early diagnosis and tr

关 键 词：甲状腺功能亢进症 妊娠 子代 肾功能 大鼠 

分 类 号：R581.1[医药卫生—内分泌]