木豆素对慢性不可预见温和应激小鼠的抗抑郁作用  

Antidepressant effects of cajaninstilbene acid on chronic unpredictable mild stress-induced depressive mice

作　　者：张梦荻 王丽莎[1] 李晨晨 陶雪[1] 周云丰[1] 刘新民[1,2] 潘瑞乐[1] 常琪[1] ZHANG Mengdi;WANG Lisha;LI Chenchen;TAO Xue;ZHOU Yunfeng;LIU Xinmin;PAN Ruile;CHANG Qi(Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193, China;National Key Laboratory of Human Factors Engineering and the State Key Laboratory ofSpace Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]中国航天员科研训练中心人因工程重点实验室,北京100094

出　　处：《中国实验动物学报》2019年第1期85-90,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：中国医学科学院创新工程:中药资源可持续利用关键技术和新药研发(2016-I2M-1-012)~~

摘　　要：目的观察木豆素于抗抑郁方面的作用,并初步探讨其可能的作用机制。方法采用小鼠慢性不可预见温和应激模型(chronic unpredictable mild stress,CUMS),进行糖水偏好实验评价经木豆素处理的小鼠的快感缺失情况,采用酶联免疫吸附测定法测定小鼠血清皮质酮的含量,采用LC-MS/MS法检测小鼠皮层和海马中多种神经递质的含量。结果木豆素可以逆转CUMS引起的糖水偏爱指数降低和血清皮质酮水平升高。并且与正常组相比,模型组小鼠皮层和海马中相关神经递质的含量发生了显著的改变,而木豆素给药对于CUMS小鼠体内相关神经递质具有明显的调节作用。结论木豆素可能通过降低血清皮质酮水平和调节脑内神经递质来实现抗抑郁作用。Objective Cajaninstilbene acid comprises a relatively high proportion of the stilbene in pigeon pea leaves and has significant anti-inflammatory and neuroprotective effects. This study aimed to observe the antidepressant effect of cajaninstilbene acid and explore its possible mechanism. MethodsA mouse model of depression was established by chronic unpredictable mild stress (CUMS) and were treated with cajaninstilbene acid. A sucrose preference test was used to evaluate the degree of anhedonia and the serum corticosterone level was detected by ELISA. The concentrations of several neurotransmitters in the cortex and hippocampus were determined by LC-MS/MS. ResultsCajaninstilbene acid reversed the decreased sucrose preference and the increased serum corticosterone levels induced by CUMS. Compared with the control group, the levels of several neurotransmitters were significantly changed in the cortex and hippocampus of CUMS mice, regulated by cajaninstilbene acid. ConclusionsCajaninstilbene acid has antidepressant effects, which may be mediated by reducing serum corticosterone levels and regulating the levels of brain neurotransmitters.

关 键 词：木豆素 抗抑郁 慢性不可预见应激 皮质酮 神经递质 

分 类 号：Q95-33[生物学—动物学]