雷公藤甲素对肿瘤坏死因子-α介导的HCM心肌细胞生长损伤保护作用研究  

Protective Effect of Triptolide on TNF-α-Mediated Damage in the Growth of HCM Cardiomyocytes

作　　者：林芸芸[1] 宋艳玲[1] 蔡海云 顾申红[1] LIN Yunyun;SONG Yanling;CAI Haiyun;GU Shenhong(The First Affiliated Hospital of Hainan Medical College,Haikou,Hainan,China 570000;The First Clinical College,Hainan Medical College,Haikou,Hainan,China 570000)

机构地区：[1]海南医学院第一附属医院,海南海口570000 [2]海南医学院第一临床学院,海南海口570000

出　　处：《中国药业》2019年第5期5-8,共4页China Pharmaceuticals

基　　金：2015年度国家自然科学基金[81560054]

摘　　要：目的探讨雷公藤甲素对肿瘤坏死因子-α(TNF-α)介导的HCM心肌细胞生长损伤的保护作用。方法采用四唑盐(MTT)比色法检测TNF-α抑制HCM细胞生长的半数抑制浓度(IC50)及雷公藤甲素干预对HCM细胞生长抑制率的影响;采用流式细胞术检测TNF-α联合雷公藤甲素对HCM细胞凋亡率的影响;使用相应试剂盒检测细胞上清液中肌酸激酶(CK)、乳酸脱氢酶(LDH)水平。结果 TNF-α对HCM细胞具有明显的生长抑制和凋亡诱导作用,且血清CK和LDH表达明显升高;低质量浓度雷公藤甲素干预后,TNF-α对HCM细胞的生长抑制和诱导凋亡作用均减弱(P <0. 01),血清CK和LDH表达呈下降趋势。结论低质量浓度雷公藤甲素可减弱TNF-α对HCM细胞的生长抑制和凋亡诱导作用。Objective To investigate the protective effect of triptolide on TNF-α-mediated damage in the growth of HCM cardiomyocytes.Methods MTT assay was used to detect the IC50 concentration of TNF-αinhibiting the growth of HCM cells,and the effect of triptolide on inhibition rate of HCM cell growth,the flow cytometry was used to detect the effect of TNF-αcombined with triptolide on the apoptotic rate of HCM cells,and the corresponding kits were used to detect the levels of creatine kinase(CK)and lactate dehydrogenase(LDH)in cell supernatants.Results TNF-αhad significant inhibition effect on the growth of HCM cells and significant induction effect on the apoptosis of HCM cells,and the expression of serum CK and LDH was significantly increased.After intervention with low concentrations of triptolide,the effect of TNF-αon the growth inhibition and apoptosis induction of HCM cells were both weakened(P<0.01),the expression of serum CK and LDH showed a decreasing trend.Conclusion Low concentrations of triptolide can attenuate the effect of TNF-αon inhibition of growth and apoptosis induction in HCM cells.

关 键 词：雷公藤甲素 肿瘤坏死因子-Α 人心肌细胞 生长损伤 细胞试验 

分 类 号：R932[医药卫生—生药学] R285.5[医药卫生—药学]