祛风舒筋丸对佐剂关节炎大鼠免疫调节及血管内皮生长因子、细胞黏附分子-1表达的影响  

The effect of Qufengshujin pills on immunoregulation and expression of VEGF and ICAM-1 in rats with adjuvant arthritis

作　　者：张瑶[1] 屠莹[1] 胡占嵩[1] 陈丽娟[1] ZHANG Yao;TU Ying;HU Zhansong;CHEN Lijuan(Department of Pharmacy,Tianjin Chest Hospital,Tianjin 300222,China)

机构地区：[1]天津市胸科医院药剂科,天津300222

出　　处：《安徽医药》2019年第3期454-458,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究祛风舒筋丸对佐剂关节炎大鼠免疫调节作用以及对血管内皮生长因子(VEGF)与细胞黏附分子-1(ICAM-1)表达的影响。方法建立弗氏完全佐剂关节炎大鼠模型,检测体外诱导脾淋巴细胞增殖水平,血清细胞因子含量以及滑膜VEGF、ICAM-1的表达水平。结果祛风舒筋丸能显著抑制模型大鼠足趾肿胀,抑制体外诱导的T、B淋巴细胞增殖,抑制类风湿因子(RF)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)的生成,抑制VEGF、ICAM-1的表达,差异有统计学意义(P<0.05)。结论祛风舒筋丸具有较强的抗类风湿关节炎作用,其作用机制可能与抑制体外诱导的T、B淋巴细胞增殖,抑制IL-1β、IL-6及TNF-α等细胞因子生成,抑制VEGF和ICAM-1的表达水平有关。Objective To study the effect of Qufengshujin Pills on immunoregulation and expression of VEGF and ICAM-1 in rats with adjuvant arthritis.Methods The models of Freund's complete adjuvant-induced arthritis in rats were established to determine splenic lymphocyte proliferation inducted in vitro,serum cytokine content and expression of VEGF and ICAM-1 in synovial membrane.Results Qufengshujin Pills could significantly inhibit paw edema in rats,inhibit T and B lymphocytes proliferation inducted in vitro,inhibit the generation of RF,IL-1β,IL-6 and TNF-α,inhibit the expression of VEGF and ICAM-1 with statistics significance(P<0.05).Conclusion Qufengshujin Pills has a strong anti-rheumatoid arthritis effect.The mechanism of action may be related to the inhibition of T and B lymphocytes proliferation,cytokine generation of IL-1β,IL-6 and TNF-αand expression of VEGF and ICAM-1.

关 键 词：祛风舒筋丸 类风湿性关节炎 作用机制 

分 类 号：R285.5[医药卫生—中药学]