基于HPLC波长切换法对养胃宁胶囊中6种成分的质量控制研究  

Quality control for six components in Yangweining capsule based on HPLC wavelength switching method

作　　者：孙辉[1] 何胜利 SUN Hui;HE Shengli(Department of Emergency Medicine,Nanyang City Center Hospital,Nanyang,Henan 473009,China;Suicheng Pharmaceutical Co.,LTD,Xinzheng,Henan 451150,China)

机构地区：[1]南阳市中心医院急诊内科,河南南阳473009 [2]遂成药业股份有限公司,河南新郑451150

出　　处：《安徽医药》2019年第3期473-476,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立同时测定养胃宁胶囊中花旗松素、山姜素、乔松素、小豆蔻明、桤木酮和α-香附酮含量的方法。方法采用Agilent Eclipse XDB C_(18)(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液,梯度洗脱;流速:0.9 mL/min;柱温25℃。结果花旗松素、山姜素、乔松素、小豆蔻明、桤木酮和α-香附酮分别在2.18～54.50μg/mL(r=0.999 9)、7.89～197.25μg/mL(r=0.999 6)、6.57～164.25μg/mL(r=0.999 8)、4.89～122.25μg/mL(r=0.999 7)、5.76～144.00μg/mL(r=0.999 9)、1.79～44.75μg/mL(r=0.999 1)范围内线性关系良好,平均加样回收率分别为97.33%、100.18%、98.47%、97.42%、99.04%和96.99%,RSD分别为1.13%、0.59%、1.49%、1.36%、0.80%和0.95%。结论该方法简便、准确、重复性好,可为养胃宁胶囊多指标质量评价提供依据。Objective To develop an HPLC wavelength switching method for determination of taxifolin,alpinetin,pinocembrin,cardamonin,alnustone andα-cyperone in Yangweining capsule simultaneously.Method The Agilent Eclipse XDB C 18(250 mm×4.6 mm,5μm)chromatographic column was adopted.The mobile phase was methanol-0.1%phosphoric acid solution with gradient elution,at a flow rate of 0.9 mL/min.The column temperature was set at 25℃.Results The linear response range were 2.18-54.50μg/mL(r=0.999 9),7.89-197.25μg/mL(r=0.999 6),6.57-164.25μg/mL(r=0.999 8),4.89-122.25μg/mL(r=0.9997),5.76-144.00μg/mL(r=0.999 9),1.79-44.75μg/mL(r=0.999 1)of taxifolin,alpinetin,pinocembrin,cardamonin,alnustone andα-cyperone,respectively.The average recoveries and the corresponding RSD were 97.33%(RSD=1.13%),100.18%(RSD=0.59%),98.47%(RSD=1.49%),97.42%(RSD=1.36%),99.04%(RSD=0.80%),96.99%(RSD=0.95%),respectively.Conclusion The method with good reproducibility is simple and accurate,which provide a new mind for quality assess of Yangweining capsule.

关 键 词：养胃宁胶囊 波长切换法 花旗松素 山姜素 乔松素 小豆蔻明 桤木酮 Α-香附酮 

分 类 号：R286.0[医药卫生—中药学]