依托咪脂对LPS诱导的神经胶质细胞中(IL)-1β表达的影响  

Effects of Etomidate on the Expression of (IL)-1β in Glial Cells Induced by LPS

作　　者：张月顺 南锡浩 芦相玉 吕晓红 张艳丽 周旋 ZHANG Yue-shun;NAN Xi-hao;LU Xiang-yu;LYU Xiao-hong;ZHANG Yan-li;ZHOU Xuan(Department of Anesthesiology,Hongqi Hospital,Mudanjiang Medical College,Mudanjiang,Heilongjiang Province,157011 China;Department of Urology,Hongqi Hospital,Mudanjiang Medical College,Mudanjiang,Heilongjiang Province,157011 China)

机构地区：[1]牡丹江医学院附属红旗医院麻醉科,黑龙江牡丹江157011 [2]牡丹江医学院附属红旗医院泌尿外科,黑龙江牡丹江157011

出　　处：《中外医疗》2019年第1期7-9,共3页China & Foreign Medical Treatment

基　　金：黑龙江省卫生计生委科研项目:依托咪脂对LPS诱导的神经胶质细胞中IL-1β表达的影响(2017-333)

摘　　要：目的探讨分析依托咪酯对LPS诱导的神经胶质细胞中(IL)-1β表达的影响。方法该次实验探究将选取该院自2016年3—11月之间培养好的原代大鼠皮层中的神经胶质细胞作为研究对象,实验研究对象为60只并将研究对象随机平均分成两组。其中一组为研究组,另一组为对照组。研究组将对研究对象采取使用依托咪脂进行处理的方案;对照组将对研究对象采取使用甲氨蝶呤进行处理的方案。然后对研究对象使用应用免疫法来测定两组的(IL)-1β的含量。结果实验后研究组大鼠各项数据均显著高于对照组(t=12.943,15.086,12.193,16.843,P<0.05);该研究中两组大鼠IL-1β水平方面,对照组治疗前后分别为(18.4±3.9)mg/L、(15.6±3.1)mg/L,研究组治疗前后分别为(18.6±3.3)mg/L、(9.8±3)mg/L(t=15.943,P<0.05);6研究组大鼠不良反应显著低于对照组(χ~2=5.643,P<0.05)。结论与采取使用甲氨蝶呤进行处理的方案相比采取使用依托咪酯进行处理的方案对LPS诱导的神经胶质细胞中(IL)-1β表达的影响效果较好,有着更加显效的抑制效果。值得在临床上进一步推广使用。Objective To investigate the effect of etomidate on the expression of(IL)-1βin glial cells induced by LPS.Methods The experimental study selected glial cells from the primary rat cortex cultured from March to November 2016 as the research object.The experimental research object included 60 cases and were randomly divided into two groups.One group was the study group and the other group was the control group.The study group will adopt a protocol for treatment with etomidate;the control group will adopt a treatment with methotrexate for the study subjects.The subject was then tested for the amount of(IL)-1βusing the applied immunoassay.Results After the experiment,the data of the rats in the study group were significantly higher than those in the control group(t=12.943,15.086,12.193,16.843,P<0.05).In the study,the levels of IL-1βin the two groups were compared before and after treatment in the control group of(18.4±3.9)mg/L,(15.6±3.1)mg/L,before and after treatment in the study group were(18.6±3.3)mg/L,(9.8±3.6)mg/L(t=15.948,P<0.05);the adverse reactions of the study group were significantly lower than that of the control group(χ^2=5.643,P<0.05).Conclusion The treatment with etomidate was used in LPS-induced glial cell(IL)-1βcompared to the protocol treated with methotrexate.The effect of expression is better,and it has a more effective inhibitory effect.It is worth further promotion in clinical use.

关 键 词：依托咪酯 LPS 神经胶质细胞 (IL)-1β 表达 影响 

分 类 号：R5[医药卫生—内科学]