黄芩苷对副猪嗜血杆菌诱导后猪血管内皮细胞黏附分子表达的影响  

Effect of baicalin on the expression of adhesion molecules in porcine vascular endothelial cells induced by Haemophilus parasuis

作　　者：徐健峰 郭靖 刘华山 付书林 邱银生 XU Jian-feng;GUO Jing;LIU Hua-shan;FU Shu-lin;QIU Yin-sheng(School of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学动物科学与营养工程学院,湖北武汉430023

出　　处：《武汉轻工大学学报》2019年第1期17-21,共5页Journal of Wuhan Polytechnic University

基　　金：国家自然科学基金委员会(31572572);武汉轻工大学校立科研项目(2017y41)

摘　　要：为了探讨黄芩苷对副猪嗜血杆菌(HPS)诱导后猪血管内皮细胞黏附分子E-选择素(E-selectin)、细胞间黏附分子1(ICAM-1)和血管细胞黏附分子(VCAM-1)的影响,体外培养猪血管内皮细胞,接种副猪嗜血杆菌。用ELISA法检测细胞培养液中E-selectin、ICAM-1和VCAM-1释放量;用Q-RT PCR法检测血管内皮细胞内E-selectin、ICAM-1和VCAM-1的mRNA表达水平。结果表明:HPS感染猪血管内皮细胞后,细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平都极显著地上调(P <0. 01);加入药物干预后,100μg/mL的黄芩苷能极显著地抑制细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平(P <0. 01)。实验表明HPS感染猪血管内皮细胞后可以激活细胞黏附分子,黄芩苷可以抑制细胞黏附分子的表达。In order to investigate the effect of baicalin on the expression of vascular endothelial cell adhesion molecules(E-selectin,ICAM-1,VCAM-1)induced by Haemophilus parasuis(HPS),porcine vascular endothelial cells were co-cultured with Haemophilus parasuis.The amount of E-selectin,ICAM-1 and VCAM-1 in cell culture medium was detected by ELISA.The mRNA expression levels of E-selectin,ICAM-1 and VCAM-1 in vascular endothelial cells were detected by Q-RT PCR.The results showed that after HPS infection with porcine vascular endothelial cells,the release of E-selectin,ICAM-1,VCAM-1 and the expression of E-selectin,ICAM-1 and VCAM-1 at mRNA levels were significantly up-regulated(P<0.01);After adding drugs,baicalin at 100μg/mL can significantly inhibit the release of E-selectin,ICAM-1,VCAM-1 in cell culture and E-selectin,ICAM-1,VCAM-1 mRNA expression levelsin vascular endothelial cells(P<0.01).Our results showed that HPS could induce the expression of cell adhesion molecules and baicalin could inhibit the expression of cell adhesion molecules.

关 键 词：黄芩苷 副猪嗜血杆菌 猪血管内皮细胞 细胞黏附分子 

分 类 号：S853.7[农业科学—临床兽医学]