Effects of Huanglian Jiedu Decoction on Rat Gingivitis Induced by LPS  

作　　者：Fangbo ZHANG Miao XU Yi ZHANG Defeng LI Hongjie WANG Haiyu ZHAO Baolin BIAN 

机构地区：[1]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences [2]State Intellectual Property Office of the PRC

出　　处：《Medicinal Plant》2018年第5期89-92,96,共5页药用植物：英文版

基　　金：Supported by the Key Project of Chinese National Programs for Fundamental Research and Development(973 Program,2014CB543003);the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ10-018-09)

摘　　要：[Objectives] To evaluate the effects of Huanglian Jiedu Decoration( HLJD) on the treatment of rat gingivitis with excess internal heat through injecting lipopolysaccharide( LPS) in tooth-supporting tissues. [Methods]Gingival inflammation was established by LPS injection. Male SD rats were randomly divided into 7 groups: normal control group( no treatment group),solvent control group,model group( LPS injection),HLJD low dose group( 0. 25 g/kg),HLJD medium dose group( 0. 50 g/kg),HLJD high dose group( 1. 00 g/kg),and metronidazole and fenbufen capsule( MFC) group. Water intake and urine volume were observed through metabolic cage feeding; gingival tissue protein expressions were detected by LC-ESI-MS/MS method; oxidative stress-related molecules such as total superoxide dismutase( T-SOD) and hydrogen peroxidase catalase( CAT) were detected by the biochemical kit combined with a microplate reader; expressions of TSC related with energy metabolism-related signaling pathway were detected by western blotting. [Results]When rat gingivitis was injected by LPS,both water intake and urine volume were decreased,the levels of T-SOD and CAT were decreased,and the expressions of fructose-bisphosphate aldolase C,triosephosphate isomerase 1 and TSC2 were increased. Huanglian Jiedu Decoration can increase water intake and urine volume,increase the levels of T-SOD and CAT,reduce the expressions of fructose-bisphosphate aldolase C and triosephosphate isomerase 1,and inhibit the expression of energy metabolism-related pathway molecule TSC2. [Conclusions] Huanglian Jiedu Decoration can treat rat gingivitis through inhibiting energy metabolism and oxidative stress such as TSC signal pathway and glycolysis related molecules.[Objectives] To evaluate the effects of Huanglian Jiedu Decoration( HLJD) on the treatment of rat gingivitis with excess internal heat through injecting lipopolysaccharide( LPS) in tooth-supporting tissues. [Methods]Gingival inflammation was established by LPS injection. Male SD rats were randomly divided into 7 groups: normal control group( no treatment group),solvent control group,model group( LPS injection),HLJD low dose group( 0. 25 g/kg),HLJD medium dose group( 0. 50 g/kg),HLJD high dose group( 1. 00 g/kg),and metronidazole and fenbufen capsule( MFC) group. Water intake and urine volume were observed through metabolic cage feeding; gingival tissue protein expressions were detected by LC-ESI-MS/MS method; oxidative stress-related molecules such as total superoxide dismutase( T-SOD) and hydrogen peroxidase catalase( CAT) were detected by the biochemical kit combined with a microplate reader; expressions of TSC related with energy metabolism-related signaling pathway were detected by western blotting. [Results]When rat gingivitis was injected by LPS,both water intake and urine volume were decreased,the levels of T-SOD and CAT were decreased,and the expressions of fructose-bisphosphate aldolase C,triosephosphate isomerase 1 and TSC2 were increased. Huanglian Jiedu Decoration can increase water intake and urine volume,increase the levels of T-SOD and CAT,reduce the expressions of fructose-bisphosphate aldolase C and triosephosphate isomerase 1,and inhibit the expression of energy metabolism-related pathway molecule TSC2. [Conclusions] Huanglian Jiedu Decoration can treat rat gingivitis through inhibiting energy metabolism and oxidative stress such as TSC signal pathway and glycolysis related molecules.

关 键 词：Huanglian Jiedu DECORATION I EXCESS internal heat GINGIVITIS GLYCOLYSIS Energy METABOLISM OXIDATIVE stress 

分 类 号：R[医药卫生]