疏肝益阳胶囊对炔雌醚诱导大鼠生精紊乱和氧化应激的缓解作用  

Protective Effect of Shugan Yiyang Capsule against Quinestrol-induced Testicular Disturbances and Oxidative Stress in Male Rats

作　　者：李健[1] 陈耀星[2] 陈福宁 王子旭[2] 曹静[2] 董玉兰[2] 刘洋 王兴德 汪洋[1,4] LI Jian;CHEN Yaoxing;CHEN Funing;WANG Zixu;CAO Jing;DONG Yulan;LIU Yang;WANG Xingde;WANG Yang(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Changping Hospital of Integrated Chinese and Western Medicine,Beijing 102208,China;Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang,Luoyang 471003,China)

机构地区：[1]河南科技大学动物科技学院,洛阳471003 [2]中国农业大学动物医学院,北京100193 [3]北京昌平区中西医结合医院,北京102208 [4]洛阳市畜禽分子病原与免疫学重点实验室,洛阳471003

出　　处：《畜牧兽医学报》2019年第2期422-430,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发项目(2018YFD0500100);国家自然科学基金(31772761);河南省自然科学基金(162300410081;162300410067);福建省兽医中药与动物保健重点实验室2018年度开放项目;河南省教育厅高等学校重点科研项目基础研究计划(16A230004);河南科技大学高级别科研项目培育基金(2016GJB004);河南科技大学SRTP项目(2017318)

摘　　要：笔者拟探讨疏肝益阳胶囊(SGYY)对炔雌醚(QES)诱导大鼠生精紊乱和氧化应激的缓解作用及机制。将20只8周龄雄性SD大鼠随机分为4组,适应饲养1周后,进行药物灌胃处理,对照组:0.1mL生理盐水+0.1mL橄榄油;SGYY组:100 mg·kg-1 SGYY;QES组:0.1 mg·kg-1 QES;QES+SGYY组:0.1 mg·kg-1 QES+100mg·kg-1 SGYY。QES溶于橄榄油;SGYY溶于生理盐水;每天1次,连续2周。处理结束后,取睾丸、附睾、精囊腺和前列腺称重、测量长径与短径,并制备睾丸组织切片,通过HE染色,观察睾丸生精小管组织结构、生精细胞比例的变化;通过免疫组织化学方法检测睾丸生精细胞PCNA表达,Tunel法检测细胞凋亡;分离血浆,检测睾酮含量变化。取睾丸匀浆检测抗氧化酶活性。结果显示,QES处理后大鼠生殖器官的质量显著下降,附睾的精子数量显著减少;生精小管的面积、直径、生精上皮的高度和生精细胞数量均显著减少。而且,生精细胞的PCNA表达和血浆睾酮含量明显下降,Tunel阳性细胞数明显增加。睾丸SOD、GSH-Px和T-AOC活性显著下降,MDA含量显著升高。SGYY增加生殖器官质量、精子数量、睾丸睾酮含量、增殖的生精细胞数量和抗氧化酶活性,降低了MDA含量和Tunel的表达。结果表明,SGYY通过改善抗氧化功能、抑制氧化应激及促进睾酮分泌等途径增加了生精细胞数量并提高睾丸的生精功能。The study was aimed to evaluate the probable protective effect of Shugan Yiyang Capsule(SGYY,Chinese herbal extract with highly efficient antioxidant activities)against testicular toxicity and oxidative stress in adult male rats exposed to quinestrol(QES)and study the possible mechanisms underlying these effects.Twenty 8-week-old male SD rats were randomly divided into 4 groups.After 1 week of adaptation,the rats were treated with gavage once daily for 2 weeks.Control group:0.1 mL physiological saline+0.1 mL olive oil;SGYY group:100 mg·kg^-1 SGYY;QES group:0.1 mg·kg^-1 QES;QES+SGYY group:0.1 mg·kg^-1 QES+100 mg·kg^-1 SGYY.QES was dissolved in olive oil;SGYY was dissolved in physiological saline.After the treatments,the testis,epididymis,seminal vesicle and prostate were weighed,and the long and short diameters were measured.The changes in the histological structure of seminiferous tubules and the proportion of spermatogenic cells were observed in testicular tissue sections by HE staining.The expression of PCNA in spermatogenic cells of testis was detected by immunohistochemistry,and the apoptosis was detected by Tunel.The blood plasma was separated and the content of testosterone was detected.The antioxidant enzyme activity was detected in the testicular homogenate.The results showed that exposure to QES decreased the weights of reproductive organs and the sperms counts in cauda epididymis of rats.A significant decrease in the seminiferous tubular area,tubular diameter,height of germinal epithelium,number of spermatogenic cell(per seminiferous tubules)and expression of PCNA level with a significant increase in the Tunel levels were observed in the testis of experimental rats over the controls.These events were accompanied by a significant reduction in the plasma testosterone levels in QES exposed rats,indicating reduced steroidogenesis.Significant reduction in the activities of SOD,GSH-Px and T-AOC and a significant increase in MDA were also observed in the testis of QES exposed rats over the controls.Convers

关 键 词：疏肝益阳胶囊 炔雌醚 生精细胞 氧化应激 精子畸形 

分 类 号：S853.9[农业科学—临床兽医学]