microRNA-144在结核性胸腔积液诊断和疗效预测中的价值  

The value of microRNA-144 in identifying and predicting the therapeutic efficacy of tuberculous pleural effusion

作　　者：焦昕[1] 刘宁[1] Jiao Xin;Liu Ning(Department of Respiratory, Shenyang Chest Hospital, Shenyang, Liaoning, 110044, China)

机构地区：[1]沈阳市胸科医院呼吸科,辽宁沈阳110044

出　　处：《当代医学》2019年第7期47-50,共4页Contemporary Medicine

摘　　要：目的研究microRNA-144在结核性胸膜炎和恶性胸膜炎鉴别诊断中的作用,探索microRNA-144在预测结核性胸膜炎疗效中的价值。方法 2015年3月至2016年3月就诊于本院的48例结核性胸膜炎患者和40例恶性胸腔积液患者,用Trizol试剂提取胸水、血中的RNA,利用RT-PCR技术检测microRNA-144的相对表达量,分析其在结核性胸腔积液和恶性胸腔积液中表达的差异,对结核性胸腔积液患者进行胸腔穿刺抽液及标准化疗方案抗结核治疗,动态检测microRNA-144在血中表达的变化,分析其与患者治疗疗效之间的关系。结果 microRNA-144在结核性胸膜炎患者外周血清和胸腔积液中的表达量显著高于恶性胸腔积液患者,差异有统计学意义(P<0.05);结核性胸腔积液患者给与穿刺抽液及抗结核治疗后,外周血中microRNA-144的表达明显下调。结论血及胸水中microRNA-144的表达量有助于鉴别结核性胸腔积液和恶性胸腔积液,血中microRNA-144的表达变化有助于预测结核性胸膜炎疗效。Objective To investigate the value of microRNA-144 in identifying of tuberculous pleural effusion and malignant pleural effusion. To explore the predictive effect of microRNA-144 on the treatment results of tuberculous pleural effusion. Methods A total of 48 cases of patients with tuberculous pleural effusion and 40 cases of patients with malignant pleural effusion treated in our hospital from March 2015 to March 2016 were included in the study. The RNA of the blood, and pleural effusion was extracted using Trizol. The expression of microRNA-144 was assessed by RT-PCR. Patients with tuberculous pleural effusion were treated with routine treatment. The clinical curative effect reaction of the two groups were observed.The expression of microRNA-144 of serum was dynamic monitoring before and after the treatment. The relation between the expression of microRNA-144 and the clinical therapeutic efficacy was analyzed. Results The blood and pleural effusion microRNA-144 of patients with tuberculous pleural effusion was significantly higher than patients with malignant pleural effusion (P<0.05). The blood microRNA-144 was downregulated after the effective treatment of tuberculous pleural effusion. Conclusion The expression of microRNA-144 in blood and pleural effusion could be used to identify of tuberculous and malignant pleural effusion. Dynamic monitoring the changes of blood microRNA-144 could predict the clinical efficacy of tuberculous pleural effusion.

关 键 词：MicroRNA-144 结核性胸腔积液 恶性胸腔积液 鉴别诊断 疗效预测 

分 类 号：R521.7[医药卫生—内科学]