木薯耐寒性种质资源及其鉴定指标的筛选与综合评价  

Selection and Comprehensive Evaluation of Cassava Cold-resistant Germplasm Resources and Identification Indexes

作　　者：张玮[1] 易拓[1] 唐维 宋勇[1,2] ZHANG Wei;YI Tuo;TANG Wei;SONG Yong(Horticulture and Landscape College of Hunan Agricultural University, Changsha, Hunan 410128, China;Engineering ResearchCenter of Potato, Changsha, Hunan 410128, China)

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]湖南省马铃薯工程技术研究中心,湖南长沙410128

出　　处：《热带作物学报》2019年第1期1-10,共10页Chinese Journal of Tropical Crops

基　　金：现代农业产业技术体系建设专项资金资助项目(No.CARS-11-HNSY);湖南省重点研发计划资助项目(No.2017NK2054)

摘　　要：先利用田间自然低温对18个木薯种质资源进行冷害分级,从中选择强耐寒品种(F200)、中耐寒品种(20-4、52-3)、弱耐寒品种(16P、SC8)做4℃低温胁迫处理,测定其幼苗叶片相对电导率、丙二醛、可溶性糖、可溶性蛋白、保护酶活性等7个生理指标,运用主成分分析、隶属函数、相关性分析和逐步回归等方法综合评价木薯种质的耐寒性及筛选耐寒鉴定指标。结果将7个单项生理指标转化为3个相互独立的综合指标,认为测定相对电导率、可溶性糖、丙二醛和过氧化氢酶共4个生理指标可作为准确快速鉴定木薯耐寒性强弱的指标;初步建立起了木薯耐寒性评价体系,发现田间与生理试验结果一致性较高:即参试木薯种质中,最耐寒品种均是F200,最不耐寒品种是SC8。The chilling injury of 18 cassava germplasm resources was classified by natural low temperature in the field. and strong cold-tolerant cultivars (F200), medium-tolerant cultivars (20-4, 52-3), and weak coldtolerant cultivars (16P, SC8) were selected for low temperature (4℃) stress treatment. The seedling leaf relative electric conductivity, the malondialdehyde, soluble sugar, soluble protein, protective enzyme activity and other seven physiological indexes were assayed using the method of principal component analysis (PCA), and the membership function, correlation analysis and stepwise regression method were used to comprehensively assess the cold resistance and screen cold appraisal indicators. Seven single physiological indexes were converted into three independent comprehensive indexes, and it was considered that four physiological indexes including relative electrical conductivity, soluble sugar, malondialdehyde and CAT four physiological indexes could be used as the indexes to accurately and quickly identify the cold resistance of cassava. The evaluation system of cassava cold resistance was initially established, and the results of field and physiological tests were highly consistent. It was found that among the cassava germplasm tested, the most cold-resistant strain was F200 and the least cold-resistant strain was SC8.

关 键 词：木薯 耐寒性 指标筛选 综合评价 

分 类 号：S533[农业科学—作物学]