橡胶树叶柄愈伤组织乳管细胞的组织化学和分子生物学研究  

Histochemical and Molecular Characterization of Laticifer Cells in Calli Derived from Petioles of Rubber Tree

作　　者：谭德冠[1] 彭晶[1] 韩冰莹[1] 付莉莉[1] 孙雪飘[1] 张家明[1] TAN Deguan;PENG Jing;HAN Bingying;FU Lili;SUN Xuepiao;ZHANG Jiaming(Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China)

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业农村部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2019年第1期73-78,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31471561);海南省自然科学基金项目(No.318MS091)

摘　　要：橡胶树乳管分化研究主要以树皮为研究材料。本研究通过组织化学染色法、尼罗红荧光染色法、免疫组织化学法和分子生物学方法证实橡胶树叶柄来源的愈伤组织中存在乳管细胞。乳管细胞在叶柄愈伤组织中随机分布,分布模式类似橡胶树初生乳管;叶柄愈伤组织乳管细胞在发育早期时橡胶粒子较少,在发育后期时充满橡胶粒子。RT-PCR扩增出叶柄愈伤组织乳管细胞的橡胶延伸因子(REF)、小橡胶粒子蛋白(SRPP)、橡胶凝集素(hevein)和橡胶转移酶(CPT)等胶乳特异基因的转录本,经比对它们序列与所报道的橡胶树树干胶乳中表达的基因序列一致,表明橡胶树叶柄愈伤组织乳管细胞拥有树皮乳管细胞相似的功能,为叶柄愈伤组织作为一种新型的研究乳管分化的模式提供了科学依据。The trunk bark of rubber tree (Hevea brasiliensis) is usually used as the material for the study of laticifer differentiation. In this study, we confirmed the presence of laticifer cells in petiole-derived calli by histochemical staining, nile red fluorescence staining, immunohistochemistry and molecular biology. Laticifer cells in petiole-derived calli were distributed randomly, similar to the primary laticifers. They contained less rubber particles in the early developmental stage, and were full of rubber particles in the late developmental stage. The laticifer-specific genes including REF, SRPP, Hevein and CPT were confirmed to be expressed from the petiole-derived callus by RT-PCR, and their transcripts were the same as those reported in the latex of the trunk barks. These results indicated that the laticifer cells in the petiole-derived calli and in barks had similar functions, which would provide a basis for petiole-derived callus as a novel research model to study laticifer differentiation in the rubber tree.

关 键 词：橡胶树 叶柄愈伤组织 乳管细胞 乳管分化 研究模式 

分 类 号：Q949.748.5[生物学—植物学]