骨髓间充质干细胞移植联用山胡椒叶提取物可改善脊髓损伤区域的炎症反应  

Bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract attenutes inflammation after spinal cord injury

作　　者：成建平 李华 李雄杰 Cheng Jianping;Li Hua;Li Xiongjie(Second Ward of Orthopedics, Hainan Western Central Hospital, Danzhou571700, Hainan Province, China)

机构地区：[1]海南西部中心医院(上海九院海南分院)骨科二病区,海南省儋州市571700

出　　处：《中国组织工程研究》2019年第13期1975-1981,共7页Chinese Journal of Tissue Engineering Research

基　　金：海南省自然科学基金面上项目(818MS165);项目负责人:成建平~~

摘　　要：背景:研究显示,骨髓间充质干细胞积极早期治疗脊髓损伤具有很高的研究价值。由于山胡椒叶富含黄酮、皂甙等物质,具有清除自由基、抗脂质过氧化等作用。两者联用治疗脊髓损伤的研究尚属罕见。目的:观察骨髓间充质干细胞移植联合山胡椒叶提取物治疗脊髓损伤的效果,并探索其机制。方法:将52只成年SD大鼠随机分为假手术组(n=12)、脊髓损伤组(n=14)、单纯骨髓间充质干细胞组(n=13)及联合治疗组(n=13)。脊髓损伤组、单纯骨髓间充质干细胞组及联合治疗组以Allen’s打击法建立脊髓损伤模型,假手术组仅暴露脊髓,不进行Allen’s打击。造模后即刻单纯骨髓间充质干细胞组在距损伤脊髓中线0.5 mm处注射骨髓间充质干细胞1.5×10~5个,联合治疗组在注射骨髓间充质干细胞基础上,灌胃5 mg/kg山胡椒叶提取物,1次/d,连续7 d。在造模后1,5,7 d,每组各取4只大鼠进行BBB评分、脊髓细胞凋亡计数,免疫组化法检测脊髓中肿瘤坏死因子α表达。结果与结论:①与脊髓损伤组相比,单纯骨髓间充质干细胞组、联合治疗组大鼠后肢功能开始逐渐恢复,BBB评分在造模后5,7 d有所上升(P <0.05或P <0.01),联合治疗组造模后5,7 d的BBB评分明显高于单纯骨髓间充质干细胞组(P <0.05);②脊髓损伤大鼠经骨髓间充质干细胞联合山胡椒叶提取物治疗后,损伤部位的组织水肿、坏死等迹象明显减轻,凋亡细胞数量明显减少,肿瘤坏死因子α表达明显下降,上述指标均优于单纯骨髓间充质干细胞组;③结果表明,骨髓间充质干细胞移植联用胡椒叶提取物可有效治疗脊髓损伤,且其作用可能与减轻肿瘤坏死因子α介导的炎症反应有关。BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells have a high value in the early treatment of spinal cord injury.Lindera glauca leaves rich in flavonoids, saponins and other substances can scavenge free radicals and suppress lipid peroxidation. However,the combination treatment of bone marrow mesenchymal stem cells and Lindera glauca leaf extract in the spinal cord injury model is still rare.OBJECTIVE: To observe the effect and mechanism of bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract on spinal cord injury.METHODS: Fifty-two adult Sprague-Dawley rats were randomly divided into sham operation(n=12), spinal cord injury(n=14), cell transplantation(n=13) and combination treatment(n=13) groups. The spinal cord injury model was established by the Allen’s method in the rats of spinal cord injury, cell transplantation and combination treatment groups. In the sham operation group, the spinal cord was only exposed but not subjected to the Allen’s striking method. The rats in the cell transplantation and combination treatment groups were administered bone marrow mesenchymal stem cells(1.5×105) 0.5 mm away from the midline of the injured spinal cord. Moreover, the rats in the combination treatment group were then intragastrically given 5 mg/kg Lindera glauca leaf extract, once a day for 7 continuous days. At 1, 5,7 days after model establishment, 4 rats from each group were used for Basso, Beattie and Bresnahan score and spinal cord cell apoptosis counting, and the expression of tumor necrosis factor-α in the spinal cord was detected by immunohistochemistry.RESULTS AND CONCLUSION:(1) Compared with the spinal cord injury group, the hindlimb motor function was significantly restored in the rats of cell transplantation and combination treatment groups. The Basso, Beattie and Bresnahan scores in the cell transplantation and combination treatment groups were markedly higher than those in the spinal cord injury group at 5 and 7 days after modeling(P < 0.05 o

关 键 词：骨髓间充质干细胞 山胡椒叶 脊髓损伤 BBB评分 细胞凋亡 肿瘤坏死因子α 氧化应激 海南省自然科学基金项目 骨髓 间质干细胞 山胡椒属 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]