霍山石斛对肾阴虚小鼠血清IL-2、IL-6及抗氧化作用的实验研究  

Study on the Effects of Dendrobium huoshanense on serum IL-2,IL-6 and Antioxidation in Mice with Kidney-yin Deficiency

作　　者：侯燕[1] 周雪[1] 乐娜 费文婷 汤如莹 王林元[1] 张建军[1] Hou Yan;Zhou Xue;Le Na;Fei Wenting;Tang Ruying;Wang Linyuan;Zhang Jianjun(Beijing University of Chinese Medicine,Beijing 10029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界中医药》2019年第2期340-344,共5页World Chinese Medicine

基　　金：国家自然科学基金面上项目(81173569);北京中医药大学在读研究生自主课题(2018-JYB-XS20)

摘　　要：目的:研究霍山石斛对肾阴虚小鼠血清白细胞介素-2(IL-2)、IL-6及抗氧化作用的影响。方法:96只昆明雄性小鼠适应性喂养7 d后随机分为正常组,模型组,铁皮石斛组,六味地黄丸组,霍山石斛(0. 33、1、2、3 g/kg) 4个剂量组,给药14d。除正常组外,其余各组小鼠每天下午给予甲状腺素溶液160 mg/kg,建立小鼠肾阴虚模型,观察霍山石斛对肾阴虚小鼠体质量、胸腺和脾脏指数,血清IL-2、IL-6含量及肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平的影响。结果:霍山石斛各给药组均能明显缓解小鼠的阴虚状态,增加体质量(P <0. 01,P<0. 05),1、2、3 g/kg剂量组霍山石斛能提高阴虚小鼠胸腺指数和脾脏指数(P <0. 01),降低血清IL-6 (P <0. 05,P <0. 01),升高IL-2(P <0. 01),升高阴虚小鼠肝组织SOD、GSH-Px、CAT水平(P <0. 01),降低肝组织MDA水平(P <0. 05,P<0. 01),且与铁皮石斛比较,其升高SOD、CAT和降低MDA作用明显增强(P <0. 01,P <0. 05)。结论:霍山石斛具有增强阴虚状态下机体免疫调节作用和增强抗氧化能力的作用,其免疫调节作用与铁皮石斛相当,抗氧化性优于铁皮石斛。Objective:To explore the effects of dendrobium huoshanense on serum IL-2,IL-6 and antioxidation in mice with kidney-yin deficiency.Methods:A total of 96 healthy Kunming male mice were divided into normol group,model group,Dendrobium candidum group(3 g/kg),Dendrobium huoshanense(0.33 g/kg,1 g/kg,2 g/kg,and 3 g/kg)four groups with the correspondent drugs for 14 days.In addition to normal group,the mice in each group were given thyroxine solution 160 mg/kg every afternoon to establish the kidney-yin deficiency model.The changes of hair,food intake,autonomous activity in mice were observed,and the body weight,thymus index and spleen index were measured,and the level of IL-6 and IL-2 in serum,the content of SOD,GSH-Px,CAT and MDA in liver tissues were detected separately.Results:Dendrobium huoshanense can obviously alleviate the yin deficiency symptoms of mice,such as yellowing dull hair,dry stool,yellow urine,restlessness,irritability and increase the body weight(P<0.01,P<0.05).Dendrobium huoshanense could obviously relieve the deficiency of the mice,and increase the body weight(P<0.01,P<0.05).The 1 g/kg,2 g/kg and 3 g/kg Dendrobium huoshanense groups could improve the thymus index and spleen index(P<0.01),and reduce the serum IL-6(P<0.05,P<0.01),increase the level of SOD,GSH-Px and CAT in liver tissue of yin deficiency mice(P<0.01),and reduce the level of MDA in liver tissue(P<0.05,P<0.01);and the effects of increasing SOD,CAT and the MDA decreasing rate of these 3 groups are faster than that of Dendrobium candidum groups.Conclusion:Dendrobium huoshanense can enhance the immune regulation and the ability of anti-oxidation of the body with kidney-yin deficiency,and the former effect is similar to that of Dendrobium candidum and the later effect is better than that of Dendrobium candidum.

关 键 词：霍山石斛 肾阴虚 抗氧化 免疫调节 

分 类 号：R285.5[医药卫生—中药学]