电针对糖尿病周围神经病变模型大鼠外周血流灌注量及其坐骨神经NGF受体表达的影响  

Effects of Electroacupuncture on Peripheral Blood Flow and Expression of NGF Receptor in Sciatic Nerve of PDN Rats

作　　者：阮继源[1] 张文红 杨丹红[1] Ruan Jiyuan;Zhang Wenhong;Yang Danhong(Acupuncture Research,The Third Clinical Medical College,Affiliated to ZhejiangChinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学第三临床医院,杭州310053

出　　处：《世界中医药》2019年第2期354-360,共7页World Chinese Medicine

基　　金：浙江中医药科技资助项目(2014ZA062)

摘　　要：目的:通过腹腔注射链脲佐菌素(Streptozocin,STZ)建立痛性糖尿病周围神经病变(Painful Diabetic Neuropathy,PDN)模型,探讨电针对PDN的治疗作用机制。方法:采用PDN组大鼠腹腔注射STZ建立糖尿病模型,观察各组大鼠的行为反应,各大鼠的一般情况、血糖、痛阈,处死前检测大鼠周围血流灌注量,于处死后采用免疫组化法检测大鼠坐骨神经神经生长因子(Nerve Growth Factor,NGF)受体的表达,进而初步探讨电针治疗PDN大鼠的部分机制。结果:与空白组比较,模型组大鼠周围血流灌注量、NGF受体的表达明显降低,电针组大鼠血流灌注量、NGF受体的表达增加,并且电针2组较电针1组血流灌注量、NGF受体的表达增加较多。结论:电针可以提高PDN模型大鼠的痛阈,增加大鼠周围血流灌注量,电针可以增加PDN模型大鼠坐骨神经NGF受体的表达,从而对损伤的坐骨神经起到修复的作用,电针能够改善PDN模型大鼠坐骨神经的病理形态和超微结构,对神经具有保护作用。Objective:To build the Painful Diabetic Neuropathy(PDN)model by intraperitoneal injection of Streptozocin(STZ),to discuss treatment mechanism of PDN by electric acupuncture(EA)and provide theoretical basis for clinical promotion.Methods:The PDN group was intraperitoneal injected STZ to build PDN model for observing behavior reaction of each groups and general state,blood glucose and pain threshold of all rats.Before death,peripheral blood perfusion was measured.After death,expression of receptor expression of Sciatic Nerve growth factor(NGF)was detected by immunohistochemistry.Moreover,it was to investigate the mechanism of electroacupuncture in the treatment of painful diabetic peripheral neuropathy in rats.Results:After building model,blood glucose of model group is increased significantly,pain threshold was declined gradually.After treatment of EA,blood glucose was not significant changed,and pain threshold was rising after treatment for 4 weeks.8-week-treatment pain threshold was rising more than 4-weeks-treatment group.Conclusion:The treatment of EA can improve the pain threshold of PDN model rats for increasing the peripheral blood perfusion and microcirculation,can improve the expression of NGF receptors in the sciatic nerve of the PDN model rats for repairing the injured sciatic nerve,can improve the Sciatic pathological morphology and ultrastructure of PDN model rats for protecting the nerves.

关 键 词：痛性糖尿病周围神经病变 电针 周围血流灌注量 神经生长因子 大鼠 实验 模型 

分 类 号：R245.3[医药卫生—针灸推拿学]