基因编辑技术的发展及其应用  

Development and Application of Gene Editing Technology

作　　者：李文 曹俊国 曹满园 李丹丽[1] 许保增[1] LI Wen;CAO Jun-guo;CAO Man-yuan;LI Dan-li;XUBao-zeng(State Key Laboratory forMolecular Biology of Special Economic Animals,Institute of Special Wild Economic Animals andPlants,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所特种经济动物分子生物学重点实验室,长春130112

出　　处：《特产研究》2019年第1期124-128,共5页Special Wild Economic Animal and Plant Research

基　　金：国家自然科学基金面上项目(31772606);吉林省国际科技合作项目(31772606);吉林特研生物技术有限责任公司研发基金(20170015);中国农业科学院创新工程(CAAS-ASTIP-2017-ISAPS)

摘　　要：基因编辑技术是对基因组序列进行特定位点精确修饰的一种技术,是研究基因功能、基因组学、基因治疗等方面的有力工具。本文综述人工锌指核酸酶、类转录激活因子效应物核酸酶和规律性重复短回文序列簇3种技术的结构原理、作用机理,并简述目前规律性重复短回文序列簇在动物基因组编辑中的应用,希望帮助人们对基因编辑技术的发展及现状有一个全面了解。Gene editing technology is a kind of technology for precise modification of genome sequence at specific sites. It is a powerful tool for studying gene function, genomics, gene therapy and so on. In this paper, the principles and characteristics of zinc finger nuclease, transcription activator - like nuclease and CRISPR/Cas9 systemwere reviewed, and the application of CRISPR/Cas9 in animal genome editing is also briefly described. It will help people to have a comprehensive understanding of the development and current situation of gene editing technology.

关 键 词：人工锌指核酸酶 类转录激活因子效应物核酸酶 规律性重复短回文序列簇 动物基因组编辑 

分 类 号：Q503[生物学—生物化学]