外源PBZ和GA3对太子参发育过程内源IAA积累及其相关基因表达的影响  

Effects of Exogenous Paclobutrazol and GA3 Treatment on the Accumulation of Endogenous IAA and Transcriptional Level of Related Genes in the Development of Tuberous Toot of Pseudostellaria heterophylla

作　　者：郑伟 周涛[2] 江维克[2] 李军[2] 肖承鸿[2] 杨昌贵[2] 张晨[2] 龚安慧 韦德群 毕艳 ZHENG Wei;ZHOU Tao;JIANG Wei-ke;LI Jun;XIAO Cheng-hong;YANG Chang-gui;ZHANG Chen;GONG An-hui;WEI De-qun;BI Yan(Graduate School of Tianjin University of Traditional Chinese Medicine,Tianjin 300193;Guiyang University of Chinese Medicine,Guiyang 550025)

机构地区：[1]天津中医药大学研究生院,天津300193 [2]贵阳中医学院,贵阳550025

出　　处：《生物技术通报》2019年第2期46-52,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(81460579);现代农业产业技术体系建设专项(CARS-21);贵州省国内一流学科建设项目[GNYL(2017)008];国家中药标准化项目(ZYBZH-Y-ZY-45)

摘　　要：探明外源PBZ对IAA生物合成的相关基因YUCCA和GH3表达模式的影响,为太子参栽培提供理论参考。以太子参为实验材料,采用酶联免疫吸附测定法和实时荧光定量PCR技术对外源PBZ和GA3处理条件下太子参块根内源IAA的含量及其相关基因的转录表达水平的动态变化进行研究。结果显示,PBZ处理后太子参中内源IAA的含量在第1次处理后10、20和30d均高于对照组,随后则低于对照组;经GA3处理后,除20 d外,其他时期内源IAA含量较对照组高。另外,内源IAA生物合成相关基因在外源PBZ和GA3处理后第50天时块根中的相应情况显示,GH3(Unigene37777)受外源PBZ的诱导而表达量上调,GH3(Unigene43146)和GH3(Unigene43412)的表达却受到抑制,但这3个基因均受外源GA3诱导;YUCCA(Unigene49937)对外源PBZ和GA3处理呈现相反的表现形式,受PBZ诱导,而受GA3抑制。PBZ和GA3对太子参发育过程中内源IAA的积累表现为诱导,同时对合成相关基因的表达也具有不同程度的响应作用。ELISA and Real-iime quantitative PCR experiments were conducted for investigating the dynamics of endogenous IAA and transcriptional level of related genes in response to exogenous paclobutrazol and GA3 treatment on P.heterophylla.The results showed that after PBZ treatment,the content of endogenous IAA by PBZ treatment at 10 d,20 d and 30 d was higher than the control groups after the first treatment,while lower than the control groups after 30 d.The content of endogenous IAA in the tuberous root by GA3 treatment showed fluctuation,i.e.,it was always higher than the control groups in other periods except for the 20d after treatment.In addition,the expression levels of genes related to endogenous IAA biosynthesis were analyzed in tuberous roots of P.heterophylla treated at 50 d after exogenous PBZ and GA3 treatment.The results showed that the Unigene37777 was up-regulated by PBZ treatment,while the expression of Unigene43146 and Unigene43412 were inhibited and the expression was down-regulated,but the three genes were induced by exogenous GA3;however,Unigene49937 presented opposite expression pattern in the treatment of exogenous PBZ and GA3,it was induced by PBZ,but inhibited by GA3.Overall,PBZ and GA3 induced the accumulation of endogenous IAA during the development of P.heterophylla,and had varied responses to the expressions of synthesis related-genes.

关 键 词：太子参 赤霉素 多效唑 吲哚-3-乙酸 

分 类 号：S567.53[农业科学—中草药栽培]