不同类型低频电磁场抵抗失重引起的骨流失  

Different Types of Low-frequency Electromagnetic Fields Resist Bone Loss Caused by Weightlessness

作　　者：李文苑 田永辉 高玉海[1] 朱保应 葸慧荣 陈克明[1] LI Wenyuan;TIAN Yonghui;GAO Yuhai;ZHU Baoying;XI Huirong;CHEN Keming(Institute of Orthopaedics,the 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army,Lanzhou 730050,China)

机构地区：[1]中国人民解放军联勤保障部队第九四○医院骨科研究所,兰州730050

出　　处：《中国医学科学院学报》2019年第1期11-20,共10页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(81270963);国家国际科技合作专项项目(2015DFR30940)~~

摘　　要：目的研究和比较50 Hz 0. 6 m T低频脉冲电磁场(PEMFs)和50 Hz 1. 8 m T正弦交变电磁场(SEMFs)防止尾吊大鼠骨量下降的效果,为失重引起的骨流失防治提供理论参考和科学依据。方法采用尾吊模型大鼠在地面模拟微重力,将40只SD大鼠随机分为对照组、后肢悬吊(HLS)组、HLS+PEMFs组和HLS+SEMFs组4组,每组10只。HLS+PEMFs组和HLS+SEMFs组分别采用50 Hz 0. 6 m T的PEMFs和50 Hz 1. 8 m T的SEMFs两种电磁场进行治疗,每天干预90 min,4周后处死大鼠。采用双能X射线吸收测定法检测大鼠股骨和椎骨骨密度(BMD),AG-IS型生物力学仪检测生物力学强度,ELISA法检测血清骨钙素(OC)、抗酒石酸酸性磷酸酶5b (Tracp 5b)浓度、甲状旁腺激素(PTH)和环磷酸腺苷(cAMP)的含量,Micro-CT和HE染色观察骨组织微结构。结果 HLS组股骨(P=0. 000)和椎骨(P=0. 001)的BMD显著低于对照组; HLS+PEMFs组股骨(P=0. 001)和椎骨(P=0. 039)的BMD显著高于HLS组; HLS+SEMFs组股骨的BMD明显高于HLS组(P=0. 003),但椎骨的BMD与HLS组差异无统计学意义(P=0. 130); HLS+PEMFs组和HLS+SEMFs组股骨(P=0. 818)和椎骨(P=0. 614)的BMD差异均无统计学意义。HLS组股骨和椎骨的最大载荷(P=0. 000,P=0. 009)和弹性模量(P=0. 015,P=0. 009)显著低于对照组; HLS+PEMFs组股骨(P=0. 038)和椎骨(P=0. 087)的最大载荷显著高于HLS组,但弹性模量与HLS组相比差异无统计学意义(P=0. 324,P=0. 091); HLS+SEMFs组股骨和椎骨的最大值载荷(P=0. 190,P=0. 222)和弹性模量(P=0. 512,P=0. 437)与HLS组相比差异均无统计学意义。HLS+PEMFs组和HLS+SEMFs组股骨(P=0. 585,P=0. 948)和椎骨(P=0. 668,P=0. 349)的最大载荷和弹性模量差异均无统计学意义。HLS组的血清OC水平显著低于对照组(P=0. 000),HLS+PEMFs组(P=0. 000)和HLS+SEMFs组(P=0. 006)的OC水平显著高于HLS组。HLS组的血清Tracp 5b浓度显著高于对照组(P=0. 011),HLS+PEMFs组(P=0. 459)和HLS+SEMFs组(P=0. 469)与对照组相比差异无统计学意义; HLObjective To compare the effects of 50-Hz 0. 6-mT low-frequency pulsed electromagnetic fields( PEMFs) and 50-Hz 1. 8-mT sinusoidal alternating electromagnetic fields( SEMFs) in preventing bone loss in tail-suspended rats, with an attempt to improve the prevention and treatment of bone loss caused by weightlessness. Methods Tail-suspension rat models were used to simulate microgravity on the ground. Forty rats were randomly divided into four groups [control group,hindlimb-suspended( HLS) group,HLS + PEMFs group,and HLS + SEMFs group],with 10 rats in each group. In the PEMFs treatment group and SEMFs treatment group,the intervention was 90 min per day. Rats were sacrificed after four weeks. Bone mineral density( BMD) of femur and vertebra was measured by dual-energy X-ray absorptiometry and biomechanical strength by AG-IS biomechanical instrument. Serum osteocalcin( OC),tartrate-resistant acid phosphatase 5 b( Tracp 5 b),parathyroid hormone( PTH),and cyclic adenosine monophosphate( cAMP) were detected by ELISA. The microstructure of bone tissue was observed by Micro-CT and HE staining. Results The BMD of the femur( P =0. 000) and vertebrae( P = 0. 001) in the HLS group was significantly lower than in the control group;the BMD of the femurs( P = 0. 001) and vertebrae( P = 0. 039) in the HLS + PEMFs group was significantly higher than in the HLS group;the BMD of the femurs in the HLS + SEMFs group was significantly higher than in the HLS group( P = 0. 003),but the BMD of the vertebrae showed no significant difference( P = 0. 130). There was no significant difference in the BMD of the femur( P = 0. 818) and vertebrae( P = 0. 614) between the HLS +PEMFs group and the HLS + SEMFs group. The maximum load( P = 0. 000,P = 0. 009) and elastic modulus( P = 0. 015,P = 0. 009) of the femurs and vertebrae in the HLS group were significantly lower than those in the control group;the maximum load of the femur( P = 0. 038) and vertebrae( P = 0. 087) in the HLS + PEMFs group was significantly higher than that in the HLS group,but t

关 键 词：低频脉冲电磁场 正弦交变电磁场 微重力 尾吊大鼠 骨形成 骨重建 

分 类 号：Q683[生物学—生物物理学]