纳米和微米一水草酸钙对Vero细胞毒性的浓度效应  

Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells

作　　者：饶晨颖 郭达 孙新园[1] 欧阳健明[1] RAO Chen-Ying;GUO Da;SUN Xin-Yuan;OUYANG Jian-Ming(Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China)

机构地区：[1]暨南大学生物矿化与结石病防治研究所,广州510632

出　　处：《无机化学学报》2019年第3期467-476,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.81670644)资助项目

摘　　要：采用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSM)和流式细胞仪等技术比较了纳米级和微米级一水草酸钙(COM)对非洲绿猴肾上皮细胞(Vero)的毒性差异。结果显示,尺寸为100 nm和6μm的COM晶体都能引起Vero细胞活力下降、乳酸脱氢酶(LDH)释放量升高、透明质酸(HA)表达量增多、溶酶体完整性降低、线粒体膜电位下降、细胞死亡率升高和细胞周期滞留,表明这些晶体对Vero细胞都有损伤作用且呈现浓度依赖性,但纳米COM的毒性及其在细胞表面的粘附量均大于微米COM晶体。Scanning electron microscope (SEM), laser confocal microscope (LSM), flow cytometry methods were used to comparatively study the toxicity difference of nano and micron calcium oxalate monohydrate (COM) on the African green monkey kidney epithelial (Vero) cells. The results revealed that both nano and micron COM could decrease cell viability, increase LDH release amount, up-regulate HA expression, decrease lysosomal integrity, decrease mitochondrial membrane potential, increased cell death rate and arrest cell cycle, which indicated that both nano and micron COM crystals could damage Vero cells and presented concentration dependent. But nano COM exhibited higher cytotoxicity and more adhesion amount than micron COM. This study may provide insights into the damage to renal epithelial cells induced by urinary crystals and the formation mechanism of kidney stones.

关 键 词：草酸钙 尺寸效应 晶体粘附 亚细胞器损伤 

分 类 号：R69[医药卫生—泌尿科学] O614.231[医药卫生—外科学]