产油微藻基因组编辑技术研究进展  

Research progress in genome editing technologies in oleaginous microalgae

作　　者：郝夏晖 黄凤洪[1] 龚阳敏[1] HAO Xia-hui;HUANG Feng-hong;GONG Yang-min(Oil Crop Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2019年第1期144-150,共7页Chinese Journal of Oil Crop Sciences

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-2013-OCRI)

摘　　要：微藻生物量与油脂产量偏低是目前微藻油脂难以商业化生产的主要原因之一,新型基因编辑技术TALEN和CRISPR/Cas9在解析油脂合成代谢的关键基因以及进一步遗传改造产油藻株方面有巨大潜力。本文主要介绍了TALEN和CRISPR/Cas9技术的基本原理,以及它们在产油微藻中的研究进展,并展望了两种技术在研究产油微藻功能基因和构建工业株系方面的应用前景。Low biomass and lipid productivities of microalgae are main reasons for the difficulty in commercial production of microalgal oils.Newly established gene editing techniques,TALEN and CRISPR/Cas9,have great potential in analysis of key genes in lipid biosynthesis pathway and further genetic modification of oil-producing algal strains.This review not only introduced TALEN and CRISPR/Cas9 techniques and their application in microalgal research,but also elaborated their importance in gene function study of oleaginous microalgae and construction of industrial strains.

关 键 词：微藻 基因组编辑 TALEN CRISPR/Cas9 油脂 

分 类 号：Q785[生物学—分子生物学]