Usp9y基因在不同发育阶段小鼠睾丸组织和睾丸相关细胞系中的表达特征  

Expression of Usp9y gene in mouse testis at different developmental stages and testis-associated cell lines

作　　者：李蕊秀 高迎春 吴家栋 李文秀[1] 张传领 肖瑞[1] LI Ruixiu;GAO Yingchun;WU Jiadong;LI Wenxiu;ZHANG Chanling;XIAO Rui(Key Laboratory of Molecular Pathology,Inner Mongolia Medical University,Huhhot 010059,China;Graduate School of Inner Mongolia Medical University;Department of Pathology,Chifeng Municipal Hospital;College of Pharmacy,Inner Mongolia Medical University)

机构地区：[1]内蒙古医科大学内蒙古自治区分子病理学重点实验室,呼和浩特010059 [2]内蒙古医科大学研究生院 [3]内蒙古赤峰市医院病理科 [4]内蒙古医科大学药学院

出　　处：《山西医科大学学报》2019年第1期15-19,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(31260260;31760304);内蒙古自治区自然科学基金项目(2015MS0372);内蒙古自治"草原英才"项目

摘　　要：目的明确Usp9y基因在不同发育阶段小鼠睾丸组织和睾丸相关细胞系中的表达与定位并探究Usp9y的功能。方法利用生物信息学分析结合RT-PCR方法检测小鼠睾丸相关细胞系和C57BL/6J小鼠各个组织(子宫,睾丸,心,肝,脾,肺,肾,大脑,小脑,眼球)中Usp9y基因表达的特异性;利用q PCR法检测不同日龄小鼠睾丸中Usp9y基因的表达差异性。结果生物信息学分析结果显示Usp9y基因在成年小鼠睾丸中特异性表达(P <0. 05)。RT-PCR结果证实Usp9y在小鼠睾丸组织中特异性表达(P <0. 05)。usp9y在小鼠睾丸间质细胞(TM3)中特异性表达,在小鼠精原细胞(GC-1)和小鼠睾丸支持细胞(15P-1)中未检测到表达。q PCR结果显示相比其他日龄小鼠,18日龄的小鼠睾丸Usp9y表达显著升高(P <0. 05)。结论通过RT-PCR的方法确定Usp9y基因在TM3细胞中特异性表达;小鼠Usp9y基因在小鼠出生后18 d表达明显增高,上述结果表明Usp9y基因在精子发生早期发挥着不可替代的作用。Objective To clarify the expression and localization of Usp9y gene in mouse testis and testicular-related cell lines at different developmental stages and to explore the function of Usp9y.Methods Bioinformatics analysis combined with RT-PCR was used to detect Usp9y gene expression in mouse testis-associated cell lines and C57BL/6J mouse tissues(uterus,testis,heart,liver,spleen,lung,kidney,brain,cerebellum,eyeball).The expression of Usp9y gene in testes of mice at different developmental stages was detected by qPCR method.Results Bioinformatics analysis showed that Usp9y was specifically expressed in the testis of the adult mouse(P<0.05).RT-PCR results confirmed the specific expression.Usp9y was specifically expressed in mouse testicular leydig cell(TM3),while it was not detected in spermatogonia(GC-1)and Sertoli cells(15P-1).The qPCR results showed that the expression of Usp9y in the testis of mice at day 18 was significantly increased(P<0.05).Conclusion The specific expression of Usp9y gene can be detected in mouse testicular Leydig cells by RT-PCR method.The expression of Usp9y gene is significantly higher at the postnatal day 18,indicating that Usp9y gene may play a significant role in the early stage of spermatogenesis.

关 键 词：Usp9y基因 睾丸 间质细胞 精原细胞 睾丸支持细胞 小鼠 

分 类 号：R339.2[医药卫生—人体生理学]