不同浓度人脂肪干细胞与脂肪颗粒混合种植对裸鼠脂肪细胞增长的影响  

Effect of concentrations of human adipose-derived stem cells on fat transplantation in nude mice

作　　者：柳幸子 全红[2] 刘君君 韩晶[2] LIU Xing-zi;QUAN Hong;LIU Jun-jun;HAN Jing(School of Medicine,Tongji University,Shanghai 200092,China;Dept.of Breast Surgery,East Hospital,Tongji University,Shanghai 200120,China)

机构地区：[1]同济大学医学院,上海200092 [2]同济大学附属东方医院乳腺外科,上海200120

出　　处：《同济大学学报（医学版）》2019年第1期16-21,共6页Journal of Tongji University(Medical Science)

基　　金：中央高校基本科研业务费专项资金(22120180601)

摘　　要：目的探讨不同浓度脂肪干细胞对自体脂肪移植的作用,为临床提供理论依据。方法 BALB/c裸鼠随机分为4组(n=2),裸鼠背部两侧筋膜下分别注入0.5 mL待移植脂肪颗粒与不同浓度脂肪干细胞的混合物,脂肪干细胞浓度如下:200μL脂肪颗粒.A组1×10~6、B组1×10~5、C组1×10~4脂肪干细胞,D组(对照组)注入单纯脂肪颗粒。每2周测量脂肪团块长短径观察裸鼠背部脂肪团块生长情况。6周后处死裸鼠,取出脂肪组织,称质量,H-E染色及CD31血管免疫组化染色对脂肪组织存活、脂肪细胞形态及组织血管化状态进行评价。结果脂肪团块长短径测量显示,脂肪团块均有缩小且各组间差异无统计学意义。6周后,A、B、C、D各组之间脂肪组织质量差异均有统计学意义(P<0.01)。H-E染色提示B、C、D组脂肪细胞生长良好。CD31血管免疫组化检测显示B组微血管形成情况良好。结论脂肪干细胞浓度过高时,与脂肪组织的生长竞争导致脂肪细胞的存活率降低。受区空间容量也对脂肪干细胞混合脂肪颗粒的生长产生了影响。Objective To investigate the effect of different concentrations of human adipose-derived stem cells(ADSCs)on fat transplantation in nude mice.Methods Human ADSCs were isolated and cultured.Human fat granules were transplanted into fascia of the back of BALB/c nude mice mixed with 1×10^6(group A),1×10^5(group B),1×10^4(group C)ADSCs per 100μL fat granules;or fat granules were transplanted without ADSCs(group D),with 2 mice in each group.The growth of adipose mass in the back of nude mice was measured.After 6 weeks,the nude mice were sacrificed,the adipose tissue was removed and weighed;the H-E staining and the CD31-immunohistochemistry of adipose tissue were performed.The survival of adipose tissue,adipocyte morphology and vascularization were evaluated.Results During the observation period of 6 weeks,there were no significant differences in the size of fat mass between the groups.After 6 weeks,there were significant differences in wet weight of fat mass among groups A,B,C and D(P<0.01).H-E staining showed that adipocytes in group B,C and D grew well.CD31 immunohistochemical staining showed that microvessel formation in group B was good.Conclusion The high concentration of adipose-derived stem cells would affect the survival of adipocytes,and the growth of transplanted fat tissue.

关 键 词：脂肪干细胞 脂肪移植术 脂肪干细胞辅助脂肪移植术 脂肪颗粒 

分 类 号：R320[医药卫生—人体解剖和组织胚胎学]