白芷香豆素类化合物对乳腺癌细胞的化疗增敏作用研究  

Effects of coumarins from Angelica dahurica on chemotherapeutic sensitivity of human breast cancer cells

作　　者：董伟 汤喜兰 赵国巍 蒋且英[3] 廖正根 梁新丽 DONG Wei;TANG Xilan;ZHAO Guowei;JIANG Qieying;LIAO Zhenggen;LIANG Xinli(Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi,China;Jiangxi Provincial Key Laboratory of Drug Design and Evaluation,School of Pharmacy,Jiangxi Science&Technology Normal University,Nanchang 330013,Jiangxi,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi,China)

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室 [2]江西省药物分子设计与评价重点实验室江西科技师范大学药学院 [3]江西中医药大学

出　　处：《中国临床药理学与治疗学》2019年第2期140-146,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：江西省教育厅科技项目(170732);江西省青年科学家培养对象计划(20153BCB23019);江西中医药大学博士启动基金(2015BS016);江西省卫生计生委中医药科研课题(2016A021)

摘　　要：目的:探讨5种白芷香豆素类化合物欧前胡素、异欧前胡素、氧化前胡素、白当归脑、异补骨脂素对MCF-7、MDA-MB-231细胞的化疗增敏作用。方法:体外培养MCF-7、MDA-MB-231乳腺癌细胞,分别给予不同浓度的白芷香豆素类化合物欧前胡素、异欧前胡素、氧化前胡素、白当归脑以及异补骨脂素作用72 h,采用MTT法评价其对MCF-7和MDA-MB-231细胞增殖的影响。以最大无毒浓度的欧前胡素、异欧前胡素、氧化前胡素、白当归脑以及异补骨脂素联合不同浓度的阿霉素作用72 h,采用MTT法评价上述5种白芷香豆素类化合物与化疗药物阿霉素合用后对MCF-7和MDA-MB-231细胞增殖的影响。结果:5种白芷香豆素类化合物对MCF-7细胞的增殖有抑制作用,其中以欧前胡素和白当归脑作用最为明显,二者作用于MCF-7细胞72 h的IC_(50)值分别为68.24μg/mL和54.20μg/mL;欧前胡素、异欧前胡素、氧化前胡素和异补骨脂素对MDA-MB-231细胞增殖有抑制作用,以异欧前胡素和欧前胡素作用最明显,二者作用于MDA-MB-231细胞72 h的IC_(50)值分别为44.64μg/mL和71.11μg/mL,而白当归脑作用较弱。与不同浓度的阿霉素单独组比较,不同浓度的阿霉素与欧前胡素、氧化前胡素和白当归脑合用后对MCF-7和MDA-MB-231细胞的增殖抑制作用更为明显,IC_(50)明显降低,增敏倍数增加。结论:白芷香豆素类化合物欧前胡素、氧化前胡素和白当归脑对MCF-7和MDA-MB-231乳腺癌细胞具有化疗增敏作用。AIM:To investigate the effects of coumarins from Angelica dahurica(imperatorin,isoimperatorin,oxypeucedanin,byakangelicol,isopsoralen)on chemotherapeutic sensitivity of two different breast cancer cell lines,MCF-7 and MDA-MB-231.METHODS:MCF-7 and MDA-MB-231 cells were treated with different concentrations of imperatorin,isoimperatorin,oxypeucedanin,byakangelicol,and isopsoralen for 72 h,respectively.The cytotoxity was evaluated by MTT assay.MCF-7 and MDA-MB-231 cells were treated with different doses of adriamycin in combination with the maximum nontoxic concentrations of imperatorin,isoimperatorin,oxypeucedanin,byakangelicol,and isopsoralen for 72 h,respectively.The cytotoxity was evaluated by MTT assay.RESULTS:Five furanocoumarins,especially imperatorin,byakangelicolin caused an antiproliferative effect in MCF-7 cells.The IC 50 values of imperatorin,byakangelicolin in MCF-7 cells at 72 h post-treatment were 68.24μg/mL and 54.20μg/mL,respectively.Imperatorin,isoimperatorin,oxypeucedanin and isopsoralen caused an antiproliferative effect in MDA-MB-231 cells.The IC 50 values of isoimperatorin,imperatorin in MDA-MB-231 cells at 72 h post-treatment were 44.64μg/mL and 71.11μg/mL respectively.Byakangelicolin had weak or no effects on the proliferation.Compared with the adriamycin group,the inhibitory effect of adriamycin combined with imperatorin,isoimperatorin and byakangelicolin on the proliferation of MCF-7 and MDA-MB-231 cells was more obvious.The value of IC 50 was decreased and the potentiation folds of adriamycin was increased.CONCLUSION:Imperatorin,oxypeucedanin and byakangelicol can inhibit proliferation of MCF-7 cells and MDA-MB-231 cells,and enhance the chemotherapeutic sensitivity.

关 键 词：白芷香豆素类化合物 乳腺癌 MCF-7细胞 MDA-MB-231细胞 化疗增敏作用 

分 类 号：R965.2[医药卫生—药理学] R737.9[医药卫生—药学]