sPD-1/sPD-L1与Th1/Th2及Th17/Treg相关细胞因子在原发免疫性血小板减少症中的研究  

Study on sPD-1/s PD-L1 and Th1/Th2 and T17/Treg related cytokine in patients with primary immune thrombocytopenia

作　　者：李姜惠子 刘洋[1] 王秀娟[1] 孙明玲[1] 庞楠楠[1] 哈力达.亚森[1] 赵芳[1] 秦玉婷[1] 郭新红[1] LI Jiang-Huizi;LIU Yang;WANG Xiu-Juan;SUN Ming-Ling;PANG Nan-Nan;HALIDA·Yasen;ZHAO Fang;QIN Yu-Ting;GUO Xin-Hong(Blood Disease Center of the First Affiliated Hospital of Xinjiang Medical University,Hematology Institute of Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]新疆医科大学第一附属医院血液病中心新疆维吾尔自治区血液病研究所,乌鲁木齐830000

出　　处：《中国免疫学杂志》2019年第4期467-470,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81360086)资助

摘　　要：目的:探讨原发免疫性血小板减少症(ITP)中s PD-1/s PD-L1的表达情况及其与Th1/Th2、Th17/Treg的平衡关系。方法:采用酶联免疫吸附试验(ELISA)分别检测35例初诊ITP患者和20例健康体检者外周血血清中s PD-1、s PD-L1、IFN-γ、IL-4、IL-17、TGF-β的水平。结果:①与正常对照组相比,ITP患者s PD-1、IFN-γ、IL-17水平升高(P=0. 008、P=0. 031、P=0. 005),IL-4、TGF-β水平降低(P=0. 028、P=0. 042),s PD-L1水平无明显变化(P=0. 107)。②Pearson相关性分析显示:s PD-1与IFN-γ呈正相关(r=0. 516,P=0. 006),与血小板计数呈负相关(r=-0. 562,P=0. 002);血小板计数与IFN-γ呈负相关(r=-0. 531,P=0. 004); s PD-1与IL-4、IL-17、TGF-β无相关性(P均>0. 05)。结论:s PD-1可能通过抑制PD-1/PD-L1负性调节通路,导致T亚群失衡,在ITP疾病中发挥一定的作用。Objective:To investigate the expression of sPD-1/sPD-L1 and its balance relationship with Th1/Th2,Th17/Treg in primary immune thrombocytopenia(ITP).Methods:The levels of sPD-1,sPD-L1,IFN-γ,IL-4,IL-17 and TGF-βwere detected in 35 patients with initial diagnosis of ITP and 20 healthy subjects by enzyme-linked immunosorbent assay(ELISA).Results:①Compared with the normal control group,the level of sPD-1,IFN-γand IL-17 in ITP patients increased(P=0.008,P=0.031,P=0.005),and IL-4 and TGF-βwere decreased(P=0.028,P=0.042),and no significant change in sPD-L1 level(P=0.107).②The correlation analysis showed that sPD-1 was positively correlated with IFN-γ(r=0.516,P=0.006),negatively correlated with platelet count(r=-0.562,P=0.002).The platelet count was negatively correlated with IFN-γ(r=-0.531,P=0.004).There was no correlation betweens PD-1 and IL-4,IL-17 and TGF-β(P>0.05).Conclusion:sPD-1 may inhibit the PD-1/PD-L1 negative regulation pathway and lead to the imbalance of T cells subgroups and play an important role in ITP disease.

关 键 词：原发免疫性血小板减少症 可溶性程序性死亡受体-1 可溶性程序性死亡配体-1 IFN-γ IL-4 IL-17 TGF-β 

分 类 号：R392.1[医药卫生—免疫学] R392.7[医药卫生—基础医学]