基于荧光定量PCR法的鸡传染性支气管炎疫苗免疫效果评价研究  

Evaluation on Immune Effect of Avian Infectious Bronchitis Vaccine by Immunofluorescence PCR

作　　者：黄庆华[1] 崔宁 马鑫鑫 陈照坤 杨少华[1] 唐娟娟 王珮珮 历成海 梁立生 古曙光 许传田[1] Huang Qinghua;Cui Ning;Ma Xinxin;Chen Zhaokun;Yang Shaohua;Tang Juanjuan;Wang Peipei;Li Chenghai;Liang Lisheng;Gu Shuguang;Xu Chuantian(Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China;College of Animal Science, Shandong Agricultural University, Taian 271018, China;Shandong Normal University, Jinan 250014, China;Nanhu Town Animal Husbandry and Veterinary Station of Donggang District, Rizhao City, Rizhao 276817, China;Xihu Town Animal Husbandry and Veterinary Station of Donggang District, Rizhao City, Rizhao 276815, China)

机构地区：[1]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]山东农业大学动物科技学院,山东泰安271018 [3]山东师范大学,山东济南250014 [4]日照市东港区南湖镇畜牧兽医站,山东日照276817 [5]日照市东港区西湖镇畜牧兽医站,山东日照276815

出　　处：《山东农业科学》2019年第2期121-123,共3页Shandong Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-41-Z10);山东省农业科学院农业科技创新工程项目(CXGC2016A10)

摘　　要：采用鸡胚传代致弱法得到一株可用于鸡传染性支气管炎疫苗的候选毒株,同时设定一个商品疫苗作为对照,采用荧光定量PCR法评价疫苗的免疫效果。结果表明,攻毒后7 d,两组试验鸡多数脏器均能检测到弱毒,部分脏器检测不到强毒,说明致弱毒株候选疫苗和商品疫苗对试验鸡有很好的保护作用。A candidate strain of avain infectious bronchitis vaccine was obtained by the method of chicken embryo transfer, and a commercial vaccine was set as the control. The immunofluorescence PCR method was used to evaluate the immune effect of the vaccine. The results showed that after 7 days of the attack, the attenuated virus could be detected in most of viscera organs in the two groups of experimental chickens, but the live virus was hardly detected in some viscera organs, which indicated that the candidate vaccine and the commercial vaccine could prevent the replication of the virulence, so they had better protective effects on the experimental chickens.

关 键 词：鸡传染性支气管炎 候选疫苗毒株 荧光定量PCR 

分 类 号：S852.657[农业科学—基础兽医学] S852.52[农业科学—兽医学]