滇黄精腐皮镰刀菌的分离鉴定  

Isolation and Identification of Fusarium solani from Polygonatum kingianum

作　　者：杨林毅 陈泽历 赖清玉 陈潞 孙雁[1] 唐朝辉 赵明富[1] 文国松[2] YANG Lin-yi;CEHN Ze-li;LAI Qing-yu;CEHN Lu;SUN Yan;TANG Zhao-hui;ZHAO Ming-fu;WEN Guo-song(Key Laboratory of Ministry of Education Agriculture Biodiversity for Plant Disease Management,Yunnan Agricultural University,Kunming 650021,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650021,China;Malong County Hongxing Breeding Farmers’ Professional Cooperatives,Qujing 655000,Yunan,China)

机构地区：[1]云南农业大学农业生物多样性控制病虫害教育部重点实验室,昆明650021 [2]云南农业大学农业与生物技术学院,昆明650021 [3]马龙县宏兴养殖农民专业合作社,云南曲靖655000

出　　处：《湖北农业科学》2019年第3期65-67,101,共4页Hubei Agricultural Sciences

摘　　要：采用组织分离法对滇黄精(Polygonatum kingianum)病害样品进行病原菌分离,进一步通过柯赫氏法则进行验证并通过形态学观察和ITS序列分析方法鉴定该病原菌。结果表明,病原菌菌株的菌丝为白色,背面淡黄色,菌丝体呈絮状、贴基生长、生长茂盛;病原菌菌株的分生孢子有两种形态,大型分生孢子呈镰刀形或柱形,小型分生孢子呈卵形或肾形;从菌株的形态学、致病性测定和ITS序列分析初步鉴定该菌为腐皮镰刀菌(Fusarium solani)。A pathogen from Polygonatum kingianum was isolated by pathogen isolation method,and identification of the pathogen was carried out by Koch's rule,morphological observation and ITS sequence analysis.The results show that the single colony mycelium of the pathogenic strain is white,the back is light yellow,and the mycelium is flocculent and flourishes.The conidia of the pathogenic strain have two forms,the large conidia are sickle-shaped or columnar,and the small conidia are oval or kidney-shaped.The pathogen was identified as Fusarium solani from the morphological,pathogenicity and ITS sequence analysis of the strains.

关 键 词：滇黄精(Polygonatum kingianum) 腐皮镰刀菌(Fusarium solani) 分离鉴定 

分 类 号：R931[医药卫生—生药学]