吴茱萸碱联合吉西他滨对舌鳞癌荷瘤裸鼠模型的抑制作用及其机制研究  

Inhibitory effect of evodiamine combined with gemcitabine on human tongue squamous cell carcinoma in nude mice and its mechanism

作　　者：刘延美 汪静 李月 赵家元 袁雪敏 祝静莉 王静[2] LIU Yan-mei;WANG Jing;LI Yue;ZHAO Jia-yuan;YUAN Xue-min;ZHU Jing-li;WANG Jing(School of Stomatology,Lanzhou University,Lanzhou,730000,China;Zhenjiang Stomatological Hospital,Zhenjiang,212002,China)

机构地区：[1]镇江市口腔医院,镇江212002 [2]兰州大学口腔医学院,兰州730000

出　　处：《天然产物研究与开发》2019年第2期204-210,215,共8页Natural Product Research and Development

基　　金：国家自然科学基金(81773942);甘肃省科技厅国际合作项目(17YF1WA165)

摘　　要：研究中药吴茱萸碱联合西药吉西他滨对舌鳞癌裸鼠皮下移植瘤的抑制作用并探讨其作用机制。建立舌鳞癌荷瘤裸鼠模型,药物干预后观察裸鼠瘤体积、进食量、进水量及对外界刺激的应激反应变化,HE染色观察移植瘤及各脏器组织病理结构,透射电镜观察肿瘤组织超微结构,Western blot及免疫组化检测NF-κB及Bcl-2家族基因蛋白表达。结果显示,较模型对照组及单药组,联合组移植瘤体积显著减小,荷瘤裸鼠的生活质量提高,肿瘤细胞病理性核分裂像数目减少,凋亡率增加,Bax、Ik-βα蛋白表达明显增加,Bcl-2、Bcl-xl、P65表达水平明显减少。吴茱萸碱联合吉西他滨抗肿瘤作用机制可能与调控NF-κB及Bcl-2家族蛋白有关。The objective of this study was to investigate the inhibitory effect of evodiamine combined with gemcitabine on subcutaneous xenograft of tongue squamous cell carcinoma in nude mice and its mechanism.The nude mice model of tongue squamous cell carcinoma was established.The tumor volume,food intake,water intake and response to external stimuli were observed after drug intervention.HE staining was used to observe the pathological structure of the transplanted tumor and various organs.Transmission electron microscope was used to observe the tumor tissue ultrastructure,western blot and immunohistochemistry were used to detect the expression of NF-κB and Bcl-2 family proteins.The results showed that compared with the model control group and the single drug group,the volume of transplanted tumors in the combined group was significantly reduced,the quality of life of the tumor-bearing nude mice was improved,the number of pathological mitotic figures in the tumor cells was decreased,and the apoptotic rate was increased.The expression levels of Bax and Ik-βαwere significantly increased,and the expression of Bcl-2,Bcl-xl and P65 were significantly decreased.The antitumor mechanism of evodiamine combined with gemcitabine may be related to the regulation of NF-κB and Bcl-2 family proteins.

关 键 词：舌鳞癌 吴茱萸碱 吉西他滨 NF-ΚB BCL-2 

分 类 号：R285[医药卫生—中药学] R78[医药卫生—中医学]