离子色谱-脉冲安培法测定芦荟多糖中7种单糖的含量  

Determination of seven monosaccharides in aloe polysaccharide using ion chromatography with pulsed amperometric detector

作　　者：乐胜锋[1] 王尉[1] 王雅萱 赵新颖[1] LE Shengfeng;WANG Wei;WANG Yaxuan;ZHAO Xinying(Beijing Centre for Physical & Chemical Analysis,Beijing Key Laboratory of Organic Materials Testing Technology & Quality Evaluation,Beijing 100094,China)

机构地区：[1]北京市理化分析测试中心有机材料检测技术与质量评价北京市重点实验室,北京100094

出　　处：《色谱》2019年第3期319-324,共6页Chinese Journal of Chromatography

基　　金：北京市科学技术研究院人才培养萌芽项目(201601);国家重大科学仪器设备开发专项(2012YQ09022908)~~

摘　　要：采用水提醇沉法提取芦荟鲜叶中的多糖,经三氟乙酸水解,利用Dionex CarboPac PA10高效阴离子色谱柱分离,氢氧化钠梯度淋洗,积分脉冲安培检测,建立了芦荟多糖中岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖7种常见单糖的测定方法。结果表明:7种单糖在线性范围内的相关系数(R^2)均高于0.997,检出限为0.007～0.024 mg/L,加标回收率为97.5%～102.5%,相对标准偏差(RSD)为0.1%～4.8%。该法简单、快速、灵敏、准确,可用于芦荟多糖中单糖含量的测定和多糖组成的研究。Aloe polysaccharide was extracted by water extraction and ethanol precipitation.It was hydrolyzed by trifluoroacetic acid,eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column,and detected using a pulsed amperometric detector.This method was used to determine the fucose,rhamnose,arabinose,galactose,glucose,mannose,and xylose in aloe polysaccharide.The results showed that the correlation coefficients(R^2)of all the seven target compounds were more than 0.997 in the linear range,and the limits of detection were between 0.007 mg/L and 0.024 mg/L.The recoveries were in the range 97.5%-102.5%with the relative standard deviations in the range 0.1%-4.8%.The method is simple,rapid,sensitive,and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.

关 键 词：离子色谱法 脉冲安培检测 芦荟多糖 单糖 

分 类 号：O658[理学—分析化学]