小鼠非酒精性脂肪性肝炎中线粒体功能的障碍  

Dysfunction of mitochondrial in mice with non-alcoholic steatohepatitis

作　　者：后文琳 潘雯 付思艺 宋美怡 王菲[2] HOU Wenlin;PAN Wen;FU Siyi;SONG Meiyi;WANG Fei(School of Life Sciences,Shanghai University,Shanghai 200444,China;Department of Gastroenterology,Tongji Hospital Affiliated to Tongji University,Shanghai 200065,China)

机构地区：[1]上海大学生命科学学院,上海200444 [2]同济大学附属同济医院消化内科,上海200065

出　　处：《上海大学学报（自然科学版）》2019年第1期1-9,共9页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金资助项目(81400635)

摘　　要：[目的]研究非酒精性脂肪性肝炎(non-alcoholic steatohepatitis, NASH)中线粒体功能的变化.[方法] C57BL/6小鼠采用胆碱蛋氨酸缺乏(methionine choline deficient, MCD)饮食诱导小鼠NASH模型,喂食胆碱蛋氨酸充足(methionine choline sufficient, MCS)组作为对照; 4周后,处死小鼠同时取血清和肝组织.生化指标检测血清丙氨酸氨基转酶(alanineaminotransferase, ALT)活性、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)活性、肝组织甘油三酯(triglyceride, TG)、总胆固醇(total cholesterol, TC)水平;观察肝组织病理学变化;实时荧光定量聚合酶链式反应(quantificational real-time polymerase chainreaction, qRT-PCR)检测小鼠肝脏组织中mtDNA/nDNA和nDNA,以及由线粒体DNA编码的的基因、线粒体DNA复制相关的基因、与小鼠肝脏中能量代谢相关的基因的表达变化.[结果]与MCS模型组相比, MCD组ALT, AST, TG水平明显升高,但TC水平降低;肝组织变性严重,产生大量脂肪空泡,小叶结构破坏严重,炎细胞浸润明显; MCD模型组线粒体生成能力、能量代谢水平和DNA修复能力显著降低.[结论]小鼠非酒精性脂肪性肝炎中线粒体功能发生障碍.[Objective]This research is to study the changes of mitochondrial function in non-alcoholic steatohepatitis(NASH).[Methods]The method is to use C57 BL/6 mice fed with choline methionine deficient(MCD)diet to induce a mouse NASH model.The choline methionine sufficient(MCS)group serves as the control group.Four weeks later,serum and liver tissue are detected when mice are sacrificed.Biochemical indicators of serum alanine aminotransferase(ALT)activity,aspartate aminotransferase(AST)activity,liver triglyceride(TG),total cholesterol(TC)levels are detected by enzymelinked immunosorbent assay.Pathological examination has also been performed.The mtDNA/nDNA and nDNA in liver tissue of mice and the expression of genes encoded by mitochondrial DNA,mitochondrial DNA replication and associated with energy metabolism in mouse liver are tested by q RT-PCR(quantificational real-time polymerase chain reaction).[Results]Compared with the MCS group,the MCD model group shows severe degeneration of liver tissue,and ALT,AST,and TG become significantly elevated,but TC levels are decreased.A large amount of fat vacuoles,severe destruction of lobular structure,and significant infiltration of inflammatory cells become increased in MCD model.In addition,the amount of mitochondrial is decreased.Furthermore,the capacity of energy metabolism and DNA repair of the MCD model group are significantly reduced.[Conclusion]Mitochondrial dysfunction occurs in mice with non-alcoholic steatohepatitis.

关 键 词：非酒精性脂肪性肝炎 线粒体 能量代谢 

分 类 号：G804.5[文化科学—运动人体科学]