3D打印肺混合细胞结构体植入兔体内研究  

Study on 3D Bioprinted Structure Lamellas of Lung Blend Cells to Implant in the Backs of Rabbits

作　　者：李跃中[1] 杨亚冬[2] 杨耿 罗涛[2] 徐怡朦 唐靓[1] 张文元[2] Li Yuezhong;Yang Yadong;Yang Geng(Institute of Health Food,Zhejiang Academy of Medical Sciences,Zhejiang 310013,China)

机构地区：[1]浙江省医学科学院保健食品研究所,杭州310013 [2]浙江省医学科学院生物工程研究所

出　　处：《医学研究杂志》2019年第2期101-104,共4页Journal of Medical Research

基　　金：浙江省医药卫生科技计划项目(2015ZDA011;2017KY299;2017KY307)

摘　　要：目的通过将三维(3D)生物打印的肺混合细胞水凝胶结构体片段植入兔背部皮下,探讨类肺组织结构体片段植入的可行性。方法采用胰蛋白酶消化法提取新生兔原代肺混合细胞。3D生物打印肺混合细胞-海藻酸钠-明胶3D水凝胶网格状结构体片段,活/死细胞双荧光染色观察细胞存活率。并将该结构体植入兔背部皮下。植入2周后,取出结构体片段,并进行组织病理学检测类肺组织结构形成情况。结果打印后的肺混合细胞在三维结构体中的存活率为83%±2%。兔肺细胞水凝胶支架体内植入2周后,可见植入物尚未降解。通过HE染色与Masson染色观察,表明植入物中肺细胞均匀分布,未见细胞变性及死亡。结论 3D生物打印的肺结构体具有再造类肺组织片段的潜力,将于基础医学和临床之间搭建桥梁,为肺脏再生打下基础。Objective Three dimensional(3D)bioprinted lung-like hydrogel structure lamellas were implanted in the rabbits′backs to discuss its feasibility of implanting lung-like structure lamellas.Methods Lung primary blend cells were extracted from lung tissue pieces of newborn rabbit by 0.25%trypsin digestion.3D structure lamella containing rabbit lung blend cells-alginate-gelatin blend was constructed using 3D bioprinting technique.Then the cells′survival rate was detected by live-dead cell double fluorescence staining.The 3D structure lamellas were implanted in the rabbits′backs.At 14 days after implantation,histopathological changes of the structure lamellas were examined by hematoxylin-eosin(HE)stain and Masson trichrome stain.Results The live/dead cell fluorescence staining results showed that the lung blend cells′survival rate was about 83%±2%after printing.The hydrogel scaffolds were not degraded at 14 days after implantation.Results of histological observation showed that the lung cells were evenly distributed in the hydrogel scaffolds,and that cell degeneration and death did not happen.Conclusion Bioprinted lung structure lamella has the potential to regenerate lung-like tissue structure.Our results demonstrated that it′s possible to build a bridge between basic medicine and clinical practice,and lay the foundation for regeneration of the lung structure lamella.

关 键 词：三维生物打印 兔原代肺混合细胞 水凝胶支架 结构体片段 植入 

分 类 号：R318.08[医药卫生—生物医学工程]