烟草过氧化氢酶基因CAT2克隆与表达特征分析  

Cloning of Catalase 2(CAT2) Gene and Study on Its Expression Pattern in Nicotiana tabacum L.

作　　者：侯含 王升平[1] 张超群 周志成 余祥文 卢燕回 贾海燕 王杰[1] 李莹 申莉莉[1] 王凤龙[1] 杨金广[1] HOU Han;WANG Shengping;ZHANG Chaoqun;ZHOU Zhicheng;YU Xiangwen;LU Yanhui;JIA Haiyan;WANG Jie;LI Ying;SHEN Lili;WANG Fenglong;YANG Jinguang(Tobacco Research Institute,Chinese Academy of Agricultural Science,Qingdao 266101,China;Jiangxi Tobacco ScienceInstitute,Nanchang 330025,China;Hunan Tobacco Science Institute,Changsha 410128,China;Sichuan Tobacco ScienceInstitute,Chengdu 615000,China;Guangxi Tobacco Monopoly Bureau,Nanning 530023,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]江西省烟草科学研究所,南昌330025 [3]湖南省烟草科学研究所,长沙410128 [4]四川省烟草科学研究所,成都615000 [5]广西壮族自治区烟草公司,南宁530023

出　　处：《中国烟草科学》2019年第1期1-8,共8页Chinese Tobacco Science

基　　金：江西省烟草公司科技项目"江西烟草病毒病绿色防控技术研究与应用"(201701002);四川省烟草公司科技项目"烟草病毒病快速检测技术的开发与应用"(SCYC201804);广西壮族自治区烟草公司科技项目"广西烟草绿色防控重大专项技术研究与推广应用"(201745000024095);湖南省烟草公司科技项目"湖南烟区主要病毒病防控关键技术研究"(201743010020088)

摘　　要：基于已有的植物Catalase 2基因(CAT2)序列设计烟草Nicotiana tabacum CAT2的特异性引物,通过RT-PCR扩增克隆获得Nicotiana tabacum var. NC89 CAT2全长mRNA序列,包含1479 bp完整的读码框(ORF),可编码492个氨基酸残基。遗传进化分析显示,烟草NC89 CAT2与N. benthamiana CAT2基因亲缘关系最近。利用Quantitative Real-time PCR技术分析了CAT2基因在NC89烟草中的组织特异性表达和其应对生物和非生物胁迫的表达差异。结果表明,CAT2基因在烟草NC89的根、茎、叶、花瓣、花萼、种子中均有表达,其中在花中相对表达量最高,其次为叶、茎、种子和根。生物和非生物胁迫处理烟株,其CAT2诱导表达分析显示,机械损伤、渗透压、低温和高温、干旱和感染PVY时CAT2表达量均上调。上述研究表明烟草CAT2基因可能参与了应对非生物和生物胁迫的过程。The full-length cDNA of Catalase 2 (CAT2) from Nicotiana tabacum var. NC89 was cloned usingspecific primers designed according to CAT2 mRNA sequences in other plants. Amino acid sequence alignment indicated that CAT2 contains a complete reading frame (ORF) of 1479 bp, which encodes 492 amino acid residues. Phylogenetic analysis showed that NC89 CAT2 shares high similarity with the CAT2 gene from N. benthamiana. Quantitative real-time PCR (qPCR) analysis revealed that CAT2 is highly expressed in petal and calyx, while less expressed in leaves, stems, seeds, and roots. The expression of NC89 CAT2 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought and PVY infection. It was suggested that CAT2 plays an important role in growth, development, and interplay of abiotic and biotic stresses in plant.

关 键 词：烟草 Catalase2 非生物胁迫 生物胁迫 

分 类 号：S572.03[农业科学—烟草工业]