炙甘草汤对衰老模型大鼠心肌及脑组织中AngⅡ和IL-1表达的影响  

Effect of Zhigancao decoction on the expression of AngⅡ and IL-1 in myocardium and brain tissue of aging model rats

作　　者：卢年华 张海霞[1] 靳国印[1] 李永民[1] 辛丹丹 苑芳琪 冯亭亭[1] Lu Nianhua;Zhang Haixia;Jin Guoyin;Li Yongmin;Xin Dandan;Yuan Fangqi;Feng Tingting(College of Traditional Chinese Medicine,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院中医学院,河北张家口075000

出　　处：《甘肃医药》2018年第12期1057-1058,1073,共3页Gansu Medical Journal

基　　金：河北省中医药管理局项目(项目编号:2015171);河北北方学院博士基金项目(项目编号:BBJJ201817)

摘　　要：目的:研究炙甘草汤对衰老大鼠心肌和脑组织中血管紧张素Ⅱ(AngⅡ)、白细胞介素1(IL-1)表达影响。方法:皮下注射D半乳糖制备衰老大鼠模型,分正常对照组、模型对照组、炙甘草汤(高、中、低剂量)组和阳性对照(维生素E)组。酶联免疫吸附法(Elisa)检测大鼠心肌及脑组织中AngⅡ和IL-1含量。结果:与正常对照组比较,模型组大鼠脑和心肌组织中AngⅡ和IL-1表达上升(P<0.05);与模型组比较,炙甘草汤各剂量组能不同程度影响衰老大鼠脑组织及心肌组织AngⅡ、IL-1的表达(P<0.05)。结论:炙甘草汤可能通过干预AngⅡ、IL-1的表达,具有抑制衰老模型大鼠心肌、脑组织炎症和潜在的类似AngⅡ拮抗剂的作用。Objective:To study the effect of Zhigancao decoction on the expression of interleukin-1(IL-1)and angiotensin Ⅱ(Ang Ⅱ)in myocardium and brain tissue of aging rats. Methods: Aging rat model were prepared by subcutaneous injection of D-galactose and divided into normal control group, model control group, Zhigancao decoction(high, medium and low dose)groups and positive control(vitamin E)group. The contents of IL-1 and AngⅡin rats myocardium and brain tissue were detected by enzyme-linked immunosorbent assay(ELISA). Results: Compared with the normal control group, the expression of IL-1 and AngⅡ in the brain and myocardial tissue of the model groups significantly increased(P<0.05).Different doses of Zhigancao decoction could affect the expression of AngⅡ and IL-1 in brain tissue and heart tissue of aging rats(P<0.05)compared with the model group. Conclusion: Zhigancao Decoction might inhibit the inflammation of myocardium and brain tissue in aging model rats and had a potential effect of similar AngⅡ antagonists.

关 键 词：炙甘草汤 衰老大鼠 IL-1 AngⅡ 

分 类 号：R259[医药卫生—中西医结合]