低剂量镉暴露刺激分化破骨细胞骨吸收活性  

Bone Resorption Activity Of Differentiated Osteoclast Stimulated By Low Dose Cadmium Exposure

作　　者：牛德群 宫诤 孙雪敏 范旭 汤同柯 付应霄[2] NIU De-qun;GONG Zheng;SUN Xue-min;FAN Xu;TANG Tong-ke;FU Ying-xiao(Second affiliated Hospital of Bengbu Medical College,Bengbu 233030,China;Biological Sciences Department of Bengbu Medical College,Bengbu 233030,China;Clinical Medicine First Department of Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院第二附属医院,安徽蚌埠233030 [2]蚌埠医学院生物科学系,安徽蚌埠233030 [3]蚌埠医学院临床医学一系,安徽蚌埠233030

出　　处：《宜春学院学报》2018年第12期65-69,共5页Journal of Yichun University

基　　金：安徽省自然科学基金项目(1508085QH172);安徽省高等学校自然科学基金项目(KJ2017A225;KJ2017A237;KJ2018A1011);国家大学生创新创业训练项目(201410367029;201610367004;201810367019);蚌埠医学院自然科学基金项目(BYKY1614ZD)

摘　　要：目的:探讨低剂量镉(Cadmium,Cd)暴露对分化破骨细胞骨骼吸收活性的影响。方法:M-CSF (25 ng/mL)+RANKL (30 ng/mL)诱导RAW264. 7细胞向破骨细胞分化,同时加入不同浓度Cd。通过实时荧光定量PCR实验,TRAP染色实验和骨吸收活性分析实验研究Cd对破骨细胞分化与活化的影响。结果:低浓度Cd能够促进破骨细胞活化相关基因Cathepsin K mRNA和MMP-9 mRNA的表达,刺激TRAP阳性细胞的分化,并增强分化破骨细胞的骨吸收活性。结论:低浓度Cd能够促进破骨细胞活化,表现为骨吸收活性升高。Objective:To investigate the effects of low dose cadmium(Cd)exposure on bone resorption activity of differentiated osteoclast.Methods:RAW264.7 cells were induced to differentiate into osteoclast by M-CSF(25 ng/mL)+RANKL(30 ng/mL),and various concentrations of Cd were added into the cells.The effects of Cd on the differentiation and activation of osteoclast were investigated by real-time quantitative PCR,TRAP staining and bone resorption assay.Results:Low concentrations of Cd could promote the expression levels of Cathepsin K mRNA and MMP-9 mRNA,stimulate the differentiation of TRAP-positive cells,and enhance the bone resorption activity of differentiated osteoclast.Conclusions:low concentrations of Cd can promote osteoclast activation,which presented that bone resorption activity is elevated.

关 键 词：镉 破骨细胞 骨吸收 

分 类 号：Q291[生物学—细胞生物学]