高效液相色谱法测定四物汤中咖啡酸含量  

Determination of caffeic acid in Siwu Decoction by high performance liquid chromatography

作　　者：张斌 刘翔元 陈思秒 张风旗 王张燕 董树国[2] ZHANG Bin;LIU Xiangyuan;CHEN Simiao;ZHANG Fengqi;WANG Zhangyan;DONG Shuguo(2015 Grade Pharmaceutical preparations Undergraduate Course,Jilin Province,132013,China;2016 Grade Medical Examination Technology Undergraduate Course,Jilin Province,132013,China;2017 Grade Pharmacy Undergraduate Course,Jilin Province,132013,China;Department of Analytical Chemistry,Jilin Medical University,Jilin City,Jilin Province,132013,China)

机构地区：[1]吉林医药学院 [2]吉林医药学院分析化学教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2019年第2期104-106,共3页Journal of Jilin Medical University

摘　　要：目的建立高效液相色谱法测定四物汤提取物中咖啡酸的含量。方法采用水提法对四物汤进行提取。色谱柱为C_(18)色谱柱(150 mm×4. 6 mm,5μm),流动相为甲醇-磷酸盐缓冲液(23∶77,pH 4. 0),检测波长323 nm。结果咖啡酸线性范围为2～10 mg/L,四物汤中咖啡酸含量为63. 52μg/g,咖啡酸平均回收率为91. 82%。结论高效液相色法可方便、快捷地对四物汤中咖啡酸含量进行测定。Objective To establish a high performance liquid chromatography (HPLC) method for the determination of caffeic acid in Siwu Decoction extract. Methods The Si wu Decoction was extracted by water extraction.The column was a C 18 column (150 mm × 4.6 mm,5 μm),and the mobile phase was methanol-phosphate buffer (23∶77,pH 4.0),and the detection wavelength was 323 nm. Results The linear range of caffeic acid was 2~10 mg/l,the caffeic acid content of Siwu Decoction was 63.52 μg/g,and the average recovery of caffeic acid was 91.82%. Conclusion The HPLC method can be used to determine the content of caffeic acid in Siwu Decoction easily and quickly.

关 键 词：高效液相色谱 四物汤 咖啡酸 

分 类 号：R284.2[医药卫生—中药学]