用于分离纯化组氨酸标签蛋白质的磁性复合纳米材料的制备  

Preparation of magnetic composite nanomaterials for separating and purifing histidine-tagged proteins

作　　者：王文静 孙宏浩 郭惠玲 WANG Wen-jing;SUN Hong-hao;GUO Hui-ling(Institute of Bioengineering and Food,Hubei University of Technology,Industrial Fermentation ofCollaborative Innovation Center in Hubei Province,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院湖北省工业发酵协同创新中心,湖北武汉430068

出　　处：《应用化工》2019年第2期294-297,共4页Applied Chemical Industry

基　　金：国家自然科学基金项目(21401051);湖北省自然科学基金(2014CFB595)

摘　　要：采用溶剂热法制备Fe_3O_4纳米粒子,通过MPS和聚丙烯酸修饰,使其表面羧基化,再与NTA-Ni^(2+)螯合,制备Fe_3O_4/MPS/PAA/NTA-Ni^(2+)磁性复合纳米粒子。利用透射电镜、激光粒度仪、红外光谱进行表征。结果表明,Fe_3O_4/MPS/PAA/NTA-Ni^(2+)磁性复合纳米粒子的形貌为球形,且较为分散,其平均水合粒径为440 nm,Zeta电位为-15.8 mV,红外光谱证实了其化学结构。对组氨酸标签蛋白的分离能力为15.6μg蛋白质/mg磁性材料,说明此金属螯合吸附剂对组氨酸标签蛋白的选择性吸附有一定的意义。Fe3O4 nanoparticles were prepared by solvent thermal method,and then functionalized by MPS and polyacrylic acid.The Fe 3O 4/MPS/PAA/NTA-Ni2+magnetic composite nanoparticles were prepared by the chelation between Fe 3O 4/MPS/PAA and NTA-Ni2+.Finally,the Fe 3O 4/MPS/PAA/NTA-Ni2+magnetic composite nanoparticles were characterized by the transmission electron microscopy,laser particle size instrument and infrared spectrum.The results showed that the morphology of Fe 3O 4/MPS/PAA/NTA-Ni2+magnetic composite nanoparticles was spherical,and the average particle size was 440 nm with the average Zeta potential of-15.8 mV.The infrared spectrum confirmed its chemical structure.Fe 3O 4/MPS/PAA/NTA-Ni2+magnetic composite nanoparticles could separate and purify the histidine-tagged protein easily,with the separation capacity of 15.6μg protein/mg magnetic materials,indicating that the metal chelating adsorbent has certain significance for protein separation.

关 键 词：FE3O4纳米粒子 组氨酸标签蛋白质 分离 

分 类 号：TQ138.13[化学工程—无机化工]