活体采卵技术在荷斯坦奶牛繁殖中的应用  

Application of Ovum Pick-up Technology in the Breeding of Holstein Dairy Cows

作　　者：黄启震 卢文瑾[1] 王丽娟 谢彤彤 付博凡 袁梦怡 王相国[1] HUANG Qi-zhen;LU Wen-jin;WANG Li-juan(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206)

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《安徽农业科学》2019年第4期107-109,共3页Journal of Anhui Agricultural Sciences

基　　金：国家重点研究计划项目(2017YFD0502200);北京市奶牛产业创新团队项目(BAIC05-2017)

摘　　要：[目的]为提高优质奶牛的繁殖力,保证低成本的情况下大量生产优质胚胎,对荷斯坦奶牛采用活体采卵的方式采集卵母细胞。[方法]将4头荷斯坦奶牛分为超排组和非超排组2组,超排组对试验牛注射FSH,非超排组对对照牛不采取任何处理。对荷斯坦奶牛进行活体采卵,并对卵母细胞数进行统计。[结果]共采集到12枚卵母细胞,经过受精培养后有8枚受精卵发育良好。[结论]采用优化的活体采卵程序并配合超排技术,能够更好地利用良种奶牛,以达到动物胚胎生产和辅助生殖的目的。[Objective]To improve the fecundity of high-quality breed cattle and produce mass of high-quality embryo with low cost,ovum was collected from Holstein dairy cows by using ovum pick-up technology.[Method]Four Holstein dairy cows were divided into two groups:superovulation group(A) and non-superovulation group(B).The dairy cows in group A was injected with FSH,no treatment was made on dairy cows in group B.The ovum was collected from Holstein dairy cows by using B ultrasonic instrument and statistics on the number of collected ovum was made.[Result]12 oocytes were obtained,and 8 fertilized eggs developed well after fertilization culture.[Conclusion]This experiment adopted an optimized ovum pick-up procedure combined with superovulation technology,which can make better use of improved dairy cows and achieve the production of animal embryo and assisted reproduction.

关 键 词：活体采卵 荷斯坦奶牛 体外受精 

分 类 号：S857.2[农业科学—临床兽医学]