百合鳞茎青霉病菌对不同药剂敏感性测定  

Sensitivity of Lily Bulb Penicillium to Different Fungicides

作　　者：杨士杰[1] 占军平 YANG Shi-jie;ZHAN Jun-ping(School of Life Science and Resources and Environment,Yichun University,Yichun,Jiangxi 336000;Jiangxi Xinlong Biological Polytron Technologies Limited Company,Yichun,Jiangxi 336000)

机构地区：[1]江西省宜春学院生命科学与资源环境学院,江西宜春336000 [2]江西新龙生物科技股份有限公司,江西宜春336000

出　　处：《安徽农业科学》2019年第4期147-149,共3页Journal of Anhui Agricultural Sciences

基　　金：地方发展研究项目基金(2015DF020)

摘　　要：[目的]研究百合鳞茎青霉病菌对不同药剂的敏感性。[方法]以嘧菌酯、醚菌酯、苯醚甲环唑、丙环唑为试剂,每个试剂均设置0.01、0.10、1.00、5.00、10.00、100.00μg/mL 6个浓度,采用菌丝生长速率法,将供试百合鳞茎青霉病菌单孢菌株在含药平板上分别进行敏感性测定。[结果]醚菌酯和丙环唑对百合鳞茎青霉病菌抑制率较高,苯醚甲环唑和嘧菌酯抑菌率相对较低。百合鳞茎青霉病菌对醚菌酯的EC_(50)小于敏感菌株标准值0.455μg/mL,百合鳞茎青霉病菌对醚菌酯敏感;百合鳞茎青霉病菌对丙环唑和苯醚甲环唑的EC_(50)分别为0.471和0.541μg/mL,均略大于敏感菌株标准值0.455μg/mL,介于0.455～0.911μg/mL,百合鳞茎青霉病菌对丙环唑、苯醚甲环唑敏感性相对较低,即病菌对这2种药剂均表现为低抗;百合鳞茎青霉病菌对嘧菌酯的EC_(50)为8.185μg/mL,大于高抗标准值3.644μg/mL,是敏感菌株标准值0.455μg/mL的17.98倍,即病菌表现为高抗。[结论]该研究为合理选用药剂、防治百合鳞茎青霉病提供参考。[Objective]To study sensitivity of lily bulb penicillium to different fungicides.[Method]Using pyrimidine,ether ester,phenyl ether methyclozole,procyclazole as reagents,each reagent was set at 0.01,0.10,1.00,5.00,10.00,100.00 μg/mL concentration,using mycelial growth rate method,the sensitivity of the single spore strain of Lilium miltiorrhiza viridis was determined on the tablet containing the drug.[Result]The inhibition rate of ether ester and procyclole was higher and that of benzene ether mezazole and pyrimidine was lower.Through regression analysis,the EC 50 of Lilium mildew was less than the standard value of 0.455 μg/mL of the sensitive strain,so the Lilium mildew was sensitive to Ethericum;The EC 50 of Lilium miltiorrhiza was 0.471 and 0.541 μg/mL,respectively,slightly higher than the standard value of 0.455 μg/mL of sensitive strains,between 0.455 and 0.911 μg/mL,Therefore,the sensitivity of Lilium miltiorrhiza to procyclazole and diphenyl ether was relatively low,that was,the bacteria showed low resistance to both of these agents;The EC 50 for pyrimidine esters was 8.185 μg/mL,which was greater than the high resistance standard value of 3.644 μg/mL.It was 17.98 times the sensitive strain standard value of 0.455 μg/mL,that was,the pathogen was highly resistant.[Conclusion]The study provide a reference for the rational selection of pharmaceutical and prevention and treatment of Lily bulb green mildew.

关 键 词：百合鳞茎青霉菌 敏感性测定 醚菌酯 嘧菌酯 苯醚甲环唑 丙环唑 

分 类 号：S436.81[农业科学—农业昆虫与害虫防治]