转录组学在牧草上的应用进展  

Research advances on transcriptomics in forage plants

作　　者：王飞 刘林波 高天歌 高鲤 包爱科[1] 王锁民[1] WANG Fei;LIU Linbo;GAO Tiange;GAO Li;BAO Aike;WANG Suomin(State Key Laboratory of Grassland Agro-ecosystems / Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs / College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,Gansu,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室/兰州大学农业农村部草牧业创新重点实验室/兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2019年第2期402-413,共12页Pratacultural Science

基　　金：国家自然科学基金(31730093;31470503)

摘　　要：牧草是发展草地畜牧业的重要基础。随着高通量测序技术的发展,转录组测序在牧草种质资源评价、重要性状形成机制以及优异基因资源挖掘、分子标记开发等方面已有较广泛的应用。本文从生长发育、生物胁迫适应机制、非生物(盐碱、极端温度、干旱、营养亏缺、重金属等)胁迫适应机理、逆境转录因子挖掘和分子标记筛选等方面对当前有关牧草转录组学研究进展进行综述并展望,以期为相关研究工作的开展提供参考。The forage plants are important basis for developing grassland animal husbandry. With the development of highthroughput sequencing technologies, more and more biologists engage in the study of transcriptome sequencing in evaluation of forage germplasm resources, formation mechanism of important traits, the exploration of excellent genes and molecular marker development. In this paper, the authors have reviewed the current researches on transcriptome of forage plants in term of plant growth and development, adaptation mechanism of biotic and abiotic stress (saline-alkali, extreme temperature, drought, nutrition deficiency and heavy metal), stress transcription factor discovery and molecular marker development. In addition, the future research trend of high-throughput sequencing technologies in forage plants was also prospected.

关 键 词：牧草 转录组学 生长发育 生物和非生物胁迫 分子标记 

分 类 号：S812[农业科学—草业科学] S540.1[农业科学—畜牧学]