茶树CsGGDPS7基因的克隆和表达分析  

Cloning and Expression Analysis of CsGGDPS7 Gene in Tea Plant(Camellia sinensis)

作　　者：王赞[1] 黄旭建 曹红利[1] 岳川[1] 郭雅玲[1] WANG Zan;HUANG Xujian;CAO Hongli;YUE Chuan;GUO Yaling(College of Horticulture,Fujian Agriculture and Forestry University,Key Laboratory of Tea Science at Universities in Fujian,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺学院茶学福建省高校重点实验室,福州350002

出　　处：《西北植物学报》2019年第1期32-41,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建农林大学科技创新专项基金(CXZX2016102);乌龙茶加工体系技术创新及茶类创衍应用研究(CXZX2017350)

摘　　要：从茶树基因组鉴定得到牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS7,以铁观音茶树为材料克隆获得全长cDNA(GenBank登录号MH891780)。CsGGDPS7序列全长1 185bp,包含1 098bp开放阅读框(ORF),编码365个氨基酸。预测结果显示在N端包含叶绿体转运肽,亚细胞定位于叶绿体中。氨基酸序列分析结果显示,具有类异戊二烯合成酶家族的5个保守域和2个特征功能域(DDXXXXD和DDXXD)。进化树结果表明与油橄榄(Olea europaea)OeGGDPS7亲缘关系最近。荧光定量检测显示,CsGGDPS7在叶片中的表达量显著高于其他组织,但花发育阶段CsGGDPS7表达量差异不显著。在乌龙茶做青过程中,CsGGDPS7在晒青阶段就快速上调表达并在三摇达到峰值。CsGGDPS1和CsGGDPS2表达也受做青调控,但CsGGDPS4不受做青调控。研究推测,CsGGDPS7可能与乌龙茶萜类香气物质合成密切相关。In this study,geranylgeranyl diphosphate synthase 7 ( CsGGDPS7) gene was identified from the tea genome.The full-length cDNA of the CsGGDPS7 was cloned from the leaves of tea cultivar ‘Tieguanyin’(GenBank accession number MH891780).The full length cDNA of CsGGDPS7 was 1185 bp,with a 1098 bp ORF,encoding 365 amino acids.Prediction results showed that CsGGDPS7 containing a N-terminal chloroplast targeting transit peptides,subcellular localization predicted that it could be located in chloroplast.Amino acid sequence analysis showed that the CsGGDPS7 had 5 conserved domains and 2 functional domains (DDXXXXD and DDXXD) of Isoprenoid-Biosyn-C1 superfamily.Phylogenetic tree analysis showed that CsGGDPS7 and Olea europaea OeGGDPS7 had the closest genetic relationship.According to qPCR detection,expression of CsGGDPS7 in leaves was significantly higher than that of other tissues.There was no significant difference in the expression of CsGGDPS7 during the flower development.During the green-making procedure of oolong tea,the expression of CsGGDPS7 was significant up-regulated rapidly from solar withering and reached its peak in the third tumbling.The expression of CsGGDPS1 and CsGGDPS2 were modulated by green-making procedure,but CsGGDPS4 did not.The result speculated that CsGGDPS7 might be related to the terpene aroma formation of oolong tea.

关 键 词：茶树 牻牛儿基牻牛儿基焦磷酸合成酶 表达分析 乌龙茶做青 

分 类 号：Q785[生物学—分子生物学] Q786