玉米o2,o16和wx基因不同聚合类型近等基因系的选育  

Breeding of Near-isogenic Lines with Different Pyramiding Types of o2,o16 and wx Genes in Maize

作　　者：王伟 王明春[2] 张开武[2] 任洪 赵德刚[1,2] 杨文鹏 WANG Wei;WANG Mingchun;ZHANG Kaiwu;REN Hong;ZHAO Degang;YANG Wenpeng(The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering,The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)/Guizhou Key Lab of Agro-Bioengineering,Institute of Agro-Bioengineering and College of Life Sciences,Guizhou University,Guiyang 550025,China;Guizhou Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院贵州省农业生物工程重点实验室/山地植物资源保护与种质创新省部共建教育部重点实验室绿色农药与农业生物工程国家重点实验室培育基地,贵阳550025 [2]贵州省农业科学院旱粮研究所,贵阳550006

出　　处：《种子》2019年第2期1-6,共6页Seed

基　　金：国家现代农业技术产业体系(CARS-02-082);贵州省高层次创新型人才培养对象项目(2014GZ 60263);贵州省科技计划项目(黔科合NY字[2014]3020号;黔科合支撑[2019]2299;黔科合重大专项字[2013]6022);贵州省自然科学基金重点项目(黔科合基础[2019]1451号);贵州省特色旱地粮食作物种质资源创新平台建设(黔科中引地[2018]4003);贵州省科研机构服务企业行动计划项目(黔科合服企[2018]4005);贵州省农业科学院院专项(黔农科院院专项[2014]008号)

摘　　要：为研究玉米高赖氨酸奥帕克-2(opaque-2,o2),奥帕克-16(opaque-16,o16)和糯性(waxy1,wx1)突变基因聚合提高营养品质的分子机理,以玉米o2,o16和wx三隐性基因聚合系QCL 8002-11(o2o2o16o16wxwx)为供体亲本,以普通玉米自交系CML 539为受体亲本,构建玉米o2、o16和wx基因不同聚合类型的近等基因系,经过多代回交和自交,利用分子标记辅助选择技术,选育出一批含有o2o16wx三隐性基因,o2o16、o2wx和o16wx双隐性基因,o2、o16和wx单隐性基因的近等基因系材料,为玉米品质的快速改良和阐明优异基因聚合提高玉米营养品质的机理提供材料基础。To dissect molecular mechanism improving the nutritional quality in maize through pyramiding high-lysine mutant genes(opaque2 and opaque16)and waxy mutant gene(waxy1),theo2,o16 and wx genes were introgressed into normal line using three recessive gene mutant(QCL8002-11,o2o2o16o16wxwx)as donor and normal line(CML 539)as a receptor.Using molecular marker-assisted selection technology,near-isogenic lines(NILs)of three recessive gene mutant(o2o16wx),double recessive gene mutants(o2o16,o2wxando16wx)and single recessive gene mutants(o2,o16andwx)were bred by multiple backcross and self-pollination.These NILs will provide materials for fast quality improvement and elucidating the molecular mechanisms underlying the nutritional quality in maize.

关 键 词：玉米 OPAQUE-2 opaque-16 近等基因系 分子标记辅助选择 

分 类 号：S513[农业科学—作物学]