黄色玉米籽粒颜色相关性状的QTL定位  

QTL Mapping of Seed Color-related Traits in Yellow Maize

作　　者：吕律 金黎明 Michael Lee 张丽萍 赵光武 LU Lü;JIN Liming;Michael Lee;ZHANG Liping;ZHAO Guangwu(College of Agriculture&Food Science,Key Laboratory of Agricultural Products Quality ImprovementTechnology in Zhejiang Province,Zhejiang Agriculture & Forestry University, Lin’an 311300,China;United States of AmericaJiangsu GAT Seeds Co.,Ltd.,Yancheng,Jiangsu 224000,China;Department of Agronomy,Iowa State University,Ames,IA, 50011,China)

机构地区：[1]浙江农林大学农业与食品科学学院浙江省农产品品质改良技术研究重点实验室,浙江临安311300 [2]江苏神农大丰种业科技有限公司,江苏盐城224055 [3]爱荷华州立大学农学系,美国爱荷华艾姆斯50011

出　　处：《种子》2019年第2期37-40,45,共5页Seed

基　　金：浙江省农业(粮食)新品种选育重大科技专项(2016C02050-9-5);浙江省自然科学基金(LY 18C130001);国家重点研发计划"七大农作物育种"重点专项(2018YFD 0100900);国家公益性行业(农业)科研专项(201303002)

摘　　要：以优良玉米自交系B 73和Mo 17构建的278个IBM Syn 10DH系为作图群体,利用Photoshop软件和Seed_Identification软件对黄色玉米籽粒颜色的RGB值、灰度值进行表型鉴定。利用单标记作图法,采用基于混合线性模型的分析软件QTL Cartographer Version 1.17,结合RGB值和灰度值进行QTL定位。结果共检测到11个QTL位点,与R值、G值、B值、灰度值紧密连锁的标记分别有1个、3个、6个、1个。本研究结果为黄色玉米籽粒颜色性状基因/QTL的精细定位和分子标记辅助育种奠定了基础。The 278 IBM Syn 10 DH lines constructed with the elite maize inbred lines B 73 and Mo 17 were used as the mapping group.The RGB values and gray values of yellow corn seed color were phenotyped by Photoshop software and Seed_Identification software.The QTL mapping was performed using a single marker mapping method and a mixed linear model based software QTL Cartographer Version 1.17 in combination with RGB values and gray values. Results a total of 11 QTL locus were detected,and there were one,three,six,and one markers closely linked to the R value,G value,B value,and gray value.The results of this study laid the foundation for fine mapping and molecular marker-assisted breeding of seed color trait genes/QTL in yellow maize.

关 键 词：黄色玉米 籽粒颜色 RGB值 灰度值 QTL定位 

分 类 号：S330.31[农业科学—作物遗传育种] S513[农业科学—农艺学]