重组UK114蛋白编码基因的序列特征及表达分析  

Sequence Characterization and Expression Analysis of Protein Encoded by UK114 Gene

作　　者：张晓红 宋彩丽 殷惠军 尹淑琴[1] 常泓 ZHANG Xiaohong;SONG Caili;YIN Huijun;YIN Shuqin;CHANG Hong(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China;Periodical Press of Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学期刊社,山西太谷030801

出　　处：《山西农业科学》2019年第3期437-441,共5页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(20090311037)

摘　　要：UK114蛋白对调节calpain活性具有重要作用,为了初步研究UK114蛋白的生物学特性,在测序的基础上,克隆和鉴定了UK114蛋白基因,分析该蛋白结构及功能预测。提取山羊肝脏中的总RNA,通过特异性引物扩增,获得UK114蛋白基因的序列,利用生物信息学方法分析了UK114蛋白的理化性质、信号肽、疏水性、糖基化、磷酸化位点,并对其二、三级结构进行预测。结果表明,克隆所得到的重组UK114蛋白基因由414个核苷酸组成,编码137个氨基酸的蛋白,其分子量约为14 ku,等电点为6.19;该蛋白氨基酸组成中丙氨酸含量最高,占15.3%,缺乏组氨酸和色氨酸;该蛋白结构中不存在信号肽,也没有糖基化位点,存在多个磷酸化位点;蛋白质二级结构中α螺旋占到了35.04%,不规则卷曲占到37.23%,β折叠占21.17%;三级结构中α螺旋位于N端,β折叠主要位于C端。该蛋白没有信号肽和糖基化位点,说明该蛋白不是分泌蛋白,不能发生糖基化,说明结构相对来说不是很稳定;有多个磷酸化位点,决定了其功能的多样性和复杂性。研究结果可为后期的蛋白质功能研究提供一定的理论依据。UK114 gene plays an important role in regulating calpain activity.To study the biological characteristics of UK114 gene,the UK114 gene was cloned and identified on the basis of sequencing,and the structure and function of UK114 gene were analyzed.The total RNA,of goat liver was amplified by specific primers to obtain the sequence of UK114 gene.The physicochemical properties,signal peptide,hydrophobicity,glycosylation and phosphorylation sites of UK114 protein were analyzed by bioinformatic approaches,and their secondary and tertiary structures were predicted.The results showed that the cloned UK114 gene was composed of 414 nucleotides and encoded 137 amino acids,the molecular qualtty of which was about 14 ku,isoelectric point 6.19.The content of Alanine was the highest in the amino acid composition of the protein,accounting for 15.3%,lacking of Histidine and Tryptophan.There were no signal peptides,no glycosylation sites,but had many phosphorylation sites in the protein structure.In the secondary structure of protein,Alpha helix was 35.04%,Random coil was 37.23%,Extend strand was 21.17%,Beta turn was 6.57%.In the tertiary structure,the Alpha helix was located at the N terminal,and the Extend strand was mainly located at the C terminal.The protein had no signal peptide and glycosylation site,which indicated that the protein was not secretory protein and cannot be glycosylated,and its structure was not very stable.There were multiple phosphorylation sites that determine the diversity and complexity of their functions.This study will provide an effective theoretical basis for the later study of its protein function.

关 键 词：UK114 基因克隆 生物信息学分析 

分 类 号：S858.27[农业科学—临床兽医学]