建立ERIC-PCR技术快速分析不同耐药性鲍曼不动杆菌的同源性  

Establishment of ERIC-PCR for rapidly analyzing homology of Acinetobacter baumannii with different drug resistances

作　　者：陈浩宁 王茹冰 史厚珍 狄逸凡 熊御云[1] 夏琳[1] 朱丽华[1] 张慧[1] Dinsh Kumar K 吴亮[2] 阴晴[1] 陈盛霞[2] 许化溪[2] CHEN Haoning;WANG Rubing;SHI Houzhen;DI Yifan;XIONG Yuyun;XIA Lin;ZHU Lihua;ZHANG Hui;DINSH Kumar K;WU Liang;YIN Qing;CHEN Shengxia;XU Huaxi(Department of Clinical Laboratory,Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212000,China;School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212013,China)

机构地区：[1]江苏大学附属医院检验科,江苏镇江212001 [2]江苏大学医学院,江苏镇江212013

出　　处：《检验医学与临床》2019年第5期577-580,584,共5页Laboratory Medicine and Clinic

基　　金：江苏省镇江市社会发展项目(SH2017024);国家寄生虫种质资源共享服务平台(平台-TDRC-22);江苏省博士后科研基金资助项目(1601002C);江苏大学大学生实践创新训练项目(201810299598W)

摘　　要：目的建立肠杆菌科基因间重复序列引物PCR技术(ERIC-PCR技术)用于分析亚胺培南耐药和亚胺培南敏感鲍曼不动杆菌同源性,为判断医院内感染发生提供一种简便的新方法。方法收集2018年3-6月江苏大学附属医院重症监护室患者痰液中分离的非重复鲍曼不动杆菌80株,其中包含50株亚胺培南耐药菌株,30株亚胺培南敏感菌株。使用细菌基因组DNA提取试剂盒提取细菌基因组DNA,PCR扩增菌株ERIC序列,Quantity One软件分析不同耐药性菌株电泳条带以判断菌株同源性。结果 50株亚胺培南耐药的鲍曼不动杆菌可分为6种基因型,其中最多的一种基因型有24株;30株亚胺培南敏感的鲍曼不动杆菌可分为20种基因型,其中最多的一种基因型仅有3株。结论该院ICU来源的亚胺培南耐药的鲍曼不动杆菌的基因型较少,提示该菌株可能发生了医院内感染。ERIC-PCR技术简单、方便,可以快速分析鲍曼不动杆菌同源性,适合于在各级医院中推广。Objective To establish the ERIC-PCR technique for analyzing the homology of Acinetobacter baumannii with imipenem-resistant and imipenem-sensitive activity so as to provide a simple and convenient method for judging the occurrence of nosocomial infection.Methods Eighty strains of non-repeated Acinetobacter baumannii isolated from the sputum in ICU of this hospital during March to June 2018 were collected,including 50 strains were imipenem resistant and 30 strains were imipenem sensitive.The bacterial genomic DNA extraction kit was used to extract the bacterial genomic DNA.The bacterial strain ERIC gene sequence was amplified by PCR.The Quantity One software was used to analyze the electrophoresis bands in different drug-resistant bacterial strains for judging its homology.Results Fifty strains of imipenem-resistant Acinetobacter baumannii could be classified into 6 genotypes,in which the maximum genotype had 24 strains;thirty strains of imipenem-sensitive Acinetobacter baumannii could be classified into 20 genotypes,in which the maximum genotypes only had 3 strains.Conclusion The imipenem-resistant Acinetobacter baumannii derived from ICU has fewer genotypes,suggesting that the strain may develop the nosocomial infection.The ERIC-PCR method is simple and convenient,can rapidly analyze the homology of Acinetobacter baumannii and is suitable for promotion in various levels of hospital.

关 键 词：鲍曼不动杆菌 肠杆菌科基因间重复序列引物PCR技术 同源性分析 院内感染 

分 类 号：R378[医药卫生—病原生物学]