化学发光法检测HCV核心抗原在丙型肝炎初期筛查中的应用研究  

Application of chemiluminescence for detecting HCV core antigen in hepatitis C initial screening

作　　者：孟繁君 焦婷婷 杨秀莲 王利新 王琦[2] MENG Fanjun;JIAO Tingting;YANG Xiulian;WANG Lixin;WANG Qi(Cerebrovascular Diseases Hospital,General Hospital of Ningxia Medical University,Yinchuan,Ningxia 750001,China;Ningxia Medical University,Yinchuan,Ningxia 750001,China)

机构地区：[1]宁夏医科大学总医院心脑血管病医院,宁夏银川750002 [2]宁夏医科大学,宁夏银川750001

出　　处：《检验医学与临床》2019年第5期588-591,共4页Laboratory Medicine and Clinic

基　　金：宁夏医科大学校级课题(XZ2016096)

摘　　要：目的探讨采用化学发光法(CLIA)检测丙型肝炎病毒核心抗原(HCV-cAg)、抗体(HCV-Ab)及HCV-RNA在丙型肝炎初期筛查中的价值。方法应用CLIA分别检测HCV-cAg和HCV-Ab,以HCVRNA定量检测结果作为参照,分别对220例标本进行检测,并对结果进行统计分析。结果 75例疑似人群的筛查标本中HCV-Ab阳性15例(20%)、HCV-cAg阳性6例(8.0%)、HCV-RNA阳性7例(9.3%)。70例确诊组标本中HCV-Ab阳性66例(94.3%)、HCV-cAg阳性62例(88.6%)、HCV-RNA阳性60例(85.7%)。75例健康人群的筛查标本中HCV-Ab阳性5例(6.7%)、HCV-cAg阳性2例(2.7%)、HCV-RNA阳性0例。HCV-Ab和HCV-cAg联合检测与HCV-RNA检测阳性率比较,差异有统计学意义(P<0.05)。结论在使用CLIA进行丙型肝炎早期筛查时,建议进行HCV-cAg与HCV-Ab联合检测。若需要确诊建议结合临床表现和作进一步的HCV-RNA定量检测。Objective To explore the value of adopting chemiluminescent immunoassay(CLIA)to detect hepatitis C virus(HCV)core antigen(HCV-cAg),antibody(HCV-Ab)and HCV-RNA in the initial screening of hepatitis C.Methods HCV-cAg and HCV-Ab were detected by using CLIA,with the results of HCV-RNA quantitative detection as reference,220 samples were detected.Then the detection results were statistically analyzed.Results In the screening samples of 75 suspected cases,15 cases(20%)were HCV-Ab positive,6 cases(8.0%)were HCV-cAg positive,and 7 cases(9.3%)were HCV-RNA positive.Among the 70 confirmed samples,66 cases(94.3%)were HCV-Ab positive,62 cases(88.6%)were HCV-cAg positive and 60 cases(85.7%)were HCV-RNA positive.Among the screening samples from 75 healthy people,5 cases(6.7%)were HCV-Ab positive,2 cases(2.7%)were HCV-cAg positive and 0 case was HCV-RNA positive.The positive rate had statistical difference between HCV-Ab and HCV-cAg combined detection with HCV-RNA detection(P<0.05).Conclusion In the early screening of hepatitis C by using the CLIA method,it is suggested to conduct the combined detection of HCV-cAg and HCV-Ab.If the definite diagnosis is needed,it is recommended to combine with clinical manifestations and further conduct HCV-RNA quantitative detection.

关 键 词：化学发光法 丙型肝炎病毒 丙型肝炎病毒核心抗原 

分 类 号：R446.1[医药卫生—诊断学]