脂蛋白脂酶基因多态性对子痫前期患病风险及血清脂质影响的研究  

Study on effect of polymorphism of lipoprotein lipase on preeclampsia prevalence risk and serum lipids

作　　者：郭晓霞[1] 梅颉 侯艳[1] 王玉珏[1] GUO Xiaoxia;MEI Jie;HOU Yan;WANG Yujue(Department of Obstetrics and Gynecology,Sichuan Provincial Academy of MedicalSciences & Sichuan Provincial People′s Hospital,Chengdu,Sichuan 610072,China)

机构地区：[1]四川省医学科学院.四川省人民医院妇产科,四川成都610072

出　　处：《检验医学与临床》2019年第5期651-656,共6页Laboratory Medicine and Clinic

摘　　要：目的探讨母体和胎儿脂蛋白代谢相关脂蛋白脂酶(LPL)基因S447X位点多态性交互作用与子痫前期的相关性,并分析这种交互作用对母体和胎儿血清脂质的影响及可能的机制。方法选择四川省人民医院产科住院子痫前期患者203例、正常妊娠孕妇210例;采集母体静脉血和胎儿脐血,分别测定血清脂质并计算血脂比值。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测脂蛋白代谢相关基因LPL S447X位点多态性,评估母体和胎儿LPL基因S447X位点多态性交互作用对子痫前期患病风险的影响。结果与正常妊娠组比较,子痫前期组母体LPL基因S447X等位基因X基因型频率降低,差异有统计学意义(P<0.05);正常妊娠组和子痫前期组胎儿S447X多态性位点基因型频率差异无统计学意义(P>0.05),表明母体LPL基因S447X突变携带者可显著降低患子痫前期的风险。以胎儿LPL基因S447X多态性位点分组,正常妊娠组S447X等位基因多态性对母体和胎儿血脂及血脂比值无明显影响;子痫前期组胎儿LPL基因S447XXX/SX基因型母体血脂TG/HDL-C、TC/HDL-C及AI均明显低于SS基因型。以母体和胎儿LPL基因S447X多态性位点联合分组,正常妊娠组中,与母体和胎儿LPL基因S447X均为SS型比较,母体和胎儿均为XX/SX型脐血的TC/HDL-C、LDL-C/HDL-C及AI差异均有统计学意义(P<0.05);子痫前期组母体和胎儿血脂及血脂比值在各组间差异均无统计学意义(P>0.05)。结论母体LPL基因S447X突变携带者可能是子痫前期的保护因素,而LPL S447X多态性位点SS基因型是子痫前期的危险因素,母体和胎儿均为SS基因型携带者其子痫前期的患病风险率将进一步增加。单独母体或胎儿基因多态性对母体和胎儿血脂及血脂比值影响较小,遗传和环境的协同作用才会对母体和胎儿血清脂质产生显著影响。Objective To study the correlation between maternal and fetal lipoprotein metabolim-associated lipoprotein lipase(LPL)gene S447X loci polymorphism interaction on preeclampsia and to analyze the effects of this interaction on maternal and fetal serum lipids and its possible mechanism.Methods Two hundreds and three inpatients with preeclampsia and210 normal pregnant women in the obstetrics of the Sichuan Provincial People′s Hospital were selected.Maternal venous blood and fetal umbilical cord blood were collected for detecting serum lipids and the blood lipid ratio was calculated.PCR-RFLP was used to detect the polymorphisms of lipoprotein metabolism related genes LPL S447X loci.The effect of maternal and fetal lipoprotein lipase(LPL)gene S447X loci polymorphism interaction on preeclampsia onset risk was evaluated.Results Compared with the normal pregnancy group,maternal LPL gene S447X allele X-type frequency in the preeclampsia group was decreased,the difference was statistically significant(P<0.05).The fetal S447X polymorphism locus genotype frequencies had no statistical difference between the normal pregnancy group and preeclampsia group(P>0.05),indicating that maternal LPL gene S447X mutation carriers could significantly reduce the risk of preeclampsia.The grouping was performed by fetal LPL gene S447X polymorphism loci,S447X allele in the normal pregnancy group had no obvious effect on maternal and fetal lipids and blood lipid ratio;maternal lipid TG/HDL-C,TC/HDL-C and AI of fetal LPL gene S447X XX/SX genotype maternal blood lipid TG/HDL-C,TC/HDL-C and AI in the preeclampsia group were significantly lower than those in the SS genotype.The grouping was performed by maternal and fetal LPL gene S447X polymorphism loci combination,in the normal pregnancy group,compared with SS type in maternal and fetal LPL gene S447X,the difference of TC/HDL-C,LDL-C/HDL-C and AI in maternal and fetal XX/SX type fetal blood had statistical significance(P<0.05).The maternal and fetal blood lipid,and blood lipids ratio in the pre

关 键 词：子痫前期 脂蛋白脂酶基因 血脂 基因多态性 

分 类 号：R714[医药卫生—妇产科学]