Box-Behnken响应面法优选心痛宁胶囊的水提工艺  

Optimization of Water Extraction Process of Xintongning Capsule by Box-Behnken Response Surface Method

作　　者：刘怡 如扎.哈布都拉 李露 王萍[1,2] 孟瑶 依孜提.帕力哈提 张丽娟 聂继红[1,2] LIU Yi;HABUDULA Ru-zha;LI Lu;WANG Ping;MENG Yao;PILIHATI Yi-zi-ti;ZHANG Li-juan;NIE Ji-hong(XinJiang Medical University,Urumqi 830011,China;Fourth Clinical Medicine School of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学,新疆乌鲁木齐830011 [2]新疆医科大学第四临床医学院,新疆乌鲁木齐830000

出　　处：《中国现代中药》2019年第2期208-212,221,共6页Modern Chinese Medicine

基　　金：新疆维吾尔自治区科技支疆项目(指令性)(201191167)

摘　　要：目的:运用Box-Behnken响应面法对心痛宁胶囊的水提取工艺进行优化。方法:在单因素试验结果的基础上,评价了丹酚酸B含量,总多糖含量和浸膏得率,选择提取次数、提取时间、浸泡时间和影响水提取过程的加水量作为主要考察因素,采用Box-Behnken响应面法设计试验。通过拟合二次多项式模型分析结果,以获得最佳水提取工艺。结果:优化水提取工艺是加入11倍的水,浸泡130 min,然后回流提取3次,每次60 min。结论:选用Box-Behnken响应面法设立的心痛宁胶囊水提工艺条件稳定可行,为后续工业化生产提供了参考。Objective:To optimize the water extraction process of Xintongning capsule by Box-Behnken response surface method.Methods:On the basis of single factor test results,salvianolic acid B content,total polysaccharide content and extract yield were evaluated,extraction times,extraction time,soaking time and the amount of water added to the water extraction process were selected as the main factors.Box-Behnken response surface method was used to design the experiment.The optimal water extraction process was obtained by fitting the results of quadratic polynomial model.Results:The optimal water extraction process was as follows:11 times of water,soaking for 130mins,and then reflux extraction for 3 times,60 mins each time.Conclusion:The water extraction process of Xintongning capsule established by Box-Behnken response surface method is stable and feasible,which provides a theoretical basis for the subsequent industrial production.

关 键 词：心痛宁胶囊 丹酚酸B 总多糖 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学] R282.71[医药卫生—中医学]