外周血HMGB1水平与不明原因复发性流产患者Th17/Treg平衡的关系  

Relationship between peripheral blood HMGB1 level and Th17/Treg balance in women with unexplained recurrent spontaneous abortion

作　　者：陶晓玲[1] 熊俊[1] 阳艳[1] 方敏[1] 李芸芸 TAO Xiaoling;XIONG Jun;YANG Yan;FANG Min;LI Yunyun(Department of Gynecologic, Hubei Maternal and Child Health Hospital, Hubei Province, Wuhan 430070, China)

机构地区：[1]湖北省妇幼保健院妇科,湖北武汉430070

出　　处：《中国医药导报》2019年第6期94-97,共4页China Medical Herald

基　　金：湖北省妇幼保健院科研课题(220933005)

摘　　要：目的探讨外周血中高迁移率族蛋白B1(HMGB1)水平与不明原因复发性流产患者辅助性T细胞17(Th17/调节性T(Treg)细胞平衡的关系。方法选择2016年1月～2018年1月在湖北省妇幼保健院(以下简称"我院")进行治疗的不明原因复发性流产患者83例为观察组,选择同期在我院进行产前检查的健康孕妇80名为对照组。入院后采集两组受试者的空腹静脉血,采用流式细胞仪检测其Th17、Treg细胞水平,并进行组间比较。采用酶联免疫吸附试验(ELISA)检测两组受试者的HMGB1、晚期糖基化终产物受体(RAGE)、白细胞介素-6(IL-6)、IL-10、IL-17、转化生长因子-β(TGF-β)水平。并探讨HMGB1与各细胞及细胞因子表达的相关性。结果观察组HMGB1、RAGE、Th17、Th17/Treg水平高于对照组,其Treg水平低于对照组,差异有高度统计学意义(P <0.01)。观察组IL-6、IL-17水平高于对照组,IL-10、TGF-β水平低于对照组,差异有高度统计学意义(P <0.01)。Pearson相关性分析显示,HMGB1与Th17、Th17/Treg、RAGE、IL-6、IL-17水平呈正相关(r=0.456、0.489、0.528、0.397、0.438,均P <0.05),与Treg、IL-10、TGF-β水平呈负相关(r=-0.552、-0.523、-0.439,均P <0.05)。结论不明原因复发性流产患者的外周血中HMGB1水平与Th17/Treg平衡间有一定的相关性,HMGB1、Th17、Treg的异常表达及Th17/Treg失衡可能参与了不明原因复发性流产的发生。Objective To investigate the relationship between the high mobility group box protein B1 (HMGB1) level in peripheral blood and the helper T cell 17 (Th17)/regulatory T (Treg) cell balance in patients with unexplained recurrent spontaneous abortion. Methods From January 2016 to January 2018, 83 patients with unexplained recurrent abortion who were treated in Hubei Maternal and Child Health Hospital ("our hospital" for short) were selected as the observation group, and 80 healthy pregnant women who underwent prenatal examination in our hospital during the same period were selected as the control group. Fasting venous blood was collected after admission. Th17 and Treg cell levels were measured by flow cytometry and compared between the two groups. The levels of Th17 and Treg cells were detected by flow cytometry and compared between groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of HMGB1, advanced glycation end product receptor (RAGE), interleukin-6 (IL-6), IL-10, IL-17, and transforming growth factor-β(TGF-β). The relationship between HMGB1 and the expression of various cells and cytokines was also investigated. Results The levels of HMGB1, RAGE, Th17 and Th17/Treg in the observation group were higher than those in the control group, and the Treg was lower than that in the control group, and the difference was highly statistically significant (P < 0.01). The levels of IL-6 and IL-17 in the observation group were higher than those in the control group, and the levels of IL-10 and TGF-β were lower than those in the control group, and the difference was highly statistically significant (P < 0.01). Pearson correlation analysis showed that HMGB1 was positively correlated with Th17, Th17/Treg, RAGE, IL-6, IL-17 levels (r = 0.456, 0.489, 0.528, 0.397, 0.438, P < 0.05). There was a negative correlation between the levels of HMGB1 and Treg, IL-10 and TGF-β(r =-0.552,-0.523,-0.439, P < 0.05). Conclusion There is a certain correlation between HMGB1 level and Th17/Treg balance in peripheral

关 键 词：高迁移率族蛋白B1 复发性流产 TH17/TREG细胞 相关性 

分 类 号：R714.21[医药卫生—妇产科学]