高效液相色谱法同时检测复方紫草油微乳主药成分含量的研究  

Simultaneous determination of main components in compound Radix arnebiae oil microemulsion by high performance liquid chromatography

作　　者：梅志辉[1] 许敏[1] 雷群芳[1] 曾嵘[1] Zhi-Hui Mei;Min Xu;Qun-Fang Lei;Rong Zeng(Department of Pharmacy,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China)

机构地区：[1]中南大学湘雅医院药学部,湖南长沙410008

出　　处：《中国现代医学杂志》2019年第5期44-48,共5页China Journal of Modern Medicine

基　　金：湖南省中医药管理局重点课题(No:201229)

摘　　要：目的建立高效液相色谱法同时检测复方紫草油微乳中黄芩苷、欧前胡素、紫草素的含量。方法采用色谱柱以0.1%磷酸溶液(A)-乙腈(B)为流动相(梯度洗脱:0～12 min,30%B;12～30 min,30%～54%B;30～45 min,54%B;45～60 min,54%～85%B);流速1.0 ml/min;检测波长254 nm;柱温30℃。结果黄芩苷、欧前胡素及紫草素分别在2.073～16.589、0.023～0.185、0.081～0.645μg范围内峰面积呈良好的线性关系(r≥0.9999);平均加样回收率分别95.79%、99.04%和97.32%,RSD分别为3.23%、3.07%和2.71%。结论该方法准确、灵敏、专属性强,为复方紫草油微乳提供多指标含量检测的质量控制方法。Objective To establish an high performance liquid chromatography(HPLC)method for measurement of Baicalin,Imperatorin and Shikonin in microemulsion of radix arnebiae oil.Methods The method was conducted on an Diamonsil C18(5 μm,250 mm×4.6 mm)with 0.1% phosphoricacid(A)-cetonitril(B)as mobile phase(gradient elution:0-12 min,30% B;12-30 min,30%-54% B;30-45 min,54% B;45-60 min,54%-85% B),low rate was 1.0 ml/min,detecting at 254 nm,column temperature 30℃.Results The linear ranges of Baicalin,Imperatorin and Shikonin were 2.073-16.589 μg,0.023-0.18 μg and 0.081-0.645 μg,respectively,r≥0.9999.The average recoveries(n=6)of the three chemicals were 95.79%,99.04%,97.32%,respectively.RSD of Baicalin,Imperatorin and Shikonin was 3.23%,3.07%,2.71%,respectively.Conclusions HPLC is accurate,sensitive and specific for quality control of microemulsion of radix arnebiae oil.

关 键 词：复方紫草油 微乳/药用制剂 色谱法 高效液相 黄芩苷 欧前胡素、紫草素/中草药 

分 类 号：R927.2[医药卫生—药学]