草鱼铜绿假单胞菌灭活疫苗发酵培养基的优化及其在发酵罐的生长试验  

Optimal fermentation medium and growth in a fermentor of Pseudomonas aeruginosa inactivated vaccine in grass carp

作　　者：孙承文[1] 赖迎迢[1] 任小波 江小燕[1] 巩华[1] 黄志斌[1] 陶家发[1] SUN Cheng-wen;LAI Ying-tiao;REN Xiao-bo;JIANG Xiao-yan;GONG Hua;HUANG Zhi-bin;TAO Jia-fa(Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510380 [2]华南农业大学兽医学院,广东广州510642

出　　处：《大连海洋大学学报》2019年第1期15-20,共6页Journal of Dalian Ocean University

基　　金：中国水产科学研究院基本科研业务费专项(2016HY-ZD0502);广东省科技计划项目(2016B020234003)

摘　　要：为培养优质的铜绿假单胞菌Pseudomonas aeruginosa菌液,通过单因素试验对草鱼铜绿假单胞菌灭活疫苗发酵培养基的氮源、碳源和磷酸盐成分进行了筛选,采用正交试验法对培养基各主要成分的用量进行了优化组合,并经过验证试验绘制出了铜绿假单胞菌JP802在优化培养基条件下的5 L发酵罐生长曲线。结果表明:草鱼赤皮病铜绿假单胞菌JP802发酵培养基中最佳氮源为蛋白胨+牛肉膏+酵母膏,最佳碳源为葡萄糖,最佳磷酸盐为磷酸氢二钾;确立了培养基的优化配方为蛋白胨10 g/L、牛肉膏5.0 g/L、酵母膏2.5 g/L、葡萄糖5.0 g/L、磷酸氢二钾0.75 g/L、氯化钠5.0 g/L,JP802菌株在此培养基中发酵14 h菌体浓度达到最大(OD_(600 nm)值为6.44)。研究表明,通过对发酵培养基的优化,可以获得更高产量的铜绿假单胞菌JP802发酵菌液。The nitrogen source, carbon source and phosphate were screened in culture medium of Pseudomonas aeruginosa inactivated vaccine in a single-factor test, and different ingredients of the medium were optimized in an orthogonal experiment, with a verification test of growth of the bacterium in a 5 L fermentor. It was found that the best fermentation of Pseudomonas aeruginosa in grass carp Ctenopharyngodon idellus with red skin disease was observed under the conditions of peptone+beef extract+yeast extract as nitrogen source, glucose as carbon source, K 2 PHO 4 as phosphate source, at a dose of peptone 10 g/L, beef extract 5.0 g/L, yeast extract 2.5 g/L, glucose 5.0 g/L , K 2HPO 4 0.75 g/L, and NaCl 5.0 g/L. The fermentation kinetics analysis revealed that the stain had the maximal cell density at 6.44 of OD 600 nm in 14 h culture, indicating that a high yield of Pseudomonas aeruginosa JP802 fermentation broth is obtained by the optimized fermentation medium.

关 键 词：草鱼 铜绿假单胞菌 发酵培养基 优化 

分 类 号：S942.5[农业科学—水产养殖]